RBRC02141

Information1

Image
BRC No.RBRC02141
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(DBH-tauGFP)36
Former Common nameB6.Cg-(DBH-tauGFP)36
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The Tg mice were backcrossed to C57BL/6J over 20 times. Line 17 (RBRC02140), line 36 (RBRC02141).
Strain descriptionThese transgenic mice express a fusion gene of bovine tau protein and GFP under the control of the human DBH promoter in typical noradrenergic and adrenergic cell groups. Line 17 and 36 were generated. The expression level of Tg in each line was following order: 17 > 36 (developer’s information).
Colony maintenance
ReferencesNeurogastroenterol. Motil., 22(3):331-e86 (2010). 19775251

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNhuman dopamine beta hydroxylase (DBH) promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
taubovine tau protein cDNAUNtauhuman dopamine beta hydroxylase (DBH) promoter

Information2

Donor DNAhuman dopamine beta-hydroxylase(DBH) promoter, rabbit beta-globin intron, bovine tau protein cDNA, jelly fish green fluorescent protein (GFP) cDNA, rabbit beta- globin poly A, SV40 early gene poly A
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurogastroenterol. Motil. 22(3):331-e86. PMID: 19775251
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)

BRC mice in Publications

No Data