Strain Data Sheet

RBRC02139

Strain Information

Image
BRC No.RBRC02139
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Cnga2<tm1Mom> Olfr151<tm25Mom> Pcdha<tm3Tyag>/TyagRbrc
Former Common nameOCNC1/M71-LacZ/BdAB
H-2 Haplotype
ES Cell lineW9.5 [129S1/Sv-Oca2<+> Tyr<+> Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University. The mutant mice were generated by crossing with CNR delta CR (BdAB) (RBRC01688) (Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University) and OCNC1 KO and M71-lacZ mice (Peter Monbaerts, The Rockefeller University).
Strain descriptionOCNC1 (Cnga2) knockout, CNR (Pcdha) delta CR mutant and M71 (Olfr151) gene ires-taulacZ knock-in triple mutant mice.
Colony maintenancePcdha: Homozygote x Homozygote; Olfr151: Homozygote x Homozygote; Cnga2: Heterozygote (female) x Wild-type (male)
References
Peripheral olfactory projections are differentially affected in mice deficient in a cyclic nucleotide-gated channel subunit.
Zheng C, Feinstein P, Bozza T, Rodriguez I, Mombaerts P
Neuron. 26(1):81-91 (2000). 10798394

The protocadherin-alpha family is involved in axonal coalescence of olfactory sensory neurons into glomeruli of the olfactory bulb in mouse.
Hasegawa S, Hamada S, Kumode Y, Esumi S, Katori S, Fukuda E, Uchiyama Y, Hirabayashi T, Mombaerts P, Yagi T
Mol Cell Neurosci, 38, 66-79 (2008). 18353676

Minigenes impart odorant receptor-specific axon guidance in the olfactory bulb.
Vassalli A, Rothman A, Feinstein P, Zapotocky M, Mombaerts P
Neuron, 35, 681-696 (2002). 12194868

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pcdhaprotocadherin alpha cluster18Pcdhatargeted mutation 3, Takeshi Yagi
loxPphage P1 loxP18loxP
IRESinternal ribosomal entry site (EMCV)9
Olfr151olfactory receptor 1519Olfr151targeted mutation 25, Peter Mombaerts
lacZbeta-galactosidase (E. coli)9
loxPphage P1 loxP9loxP
taubovine tau protein cDNA9tau
Cnga2cyclic nucleotide gated channel alpha 2XCnga2targeted mutation 1, Peter Mombaerts
loxPphage P1 loxPXloxP
loxPphage P1 loxPXloxP
neoneomycin resistance gene (E. coli)Xmouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cerebral cortex morphology(MP:0000788)

  • abnormal cingulate gyrus morphology(MP:0004167)

  • abnormal dentate gyrus morphology(MP:0000812)

  • abnormal dorsal striatum morphology(MP:0004102)

  • abnormal entorhinal cortex morphology(MP:0006300)
    • more 20 phenotypes
  • abnormal globus pallidus morphology(MP:0004081)

  • abnormal hippocampus morphology(MP:0000807)

  • abnormal lateral geniculate nucleus morphology(MP:0004165)

  • abnormal medial geniculate nucleus morphology(MP:0008930)

  • abnormal neuron physiology(MP:0004811)

  • abnormal olfactory bulb morphology(MP:0000819)

  • abnormal olfactory neuron innervation pattern(MP:0012014)

  • abnormal olfactory system physiology(MP:0001983)

  • abnormal primary motor cortex morphology(MP:0012448)

  • abnormal serotonergic neuron morphology(MP:0003877)

  • abnormal serotonin level(MP:0005322)

  • abnormal substantia nigra morphology(MP:0000836)

  • abnormal thalamus morphology(MP:0000832)

  • decreased body weight(MP:0001262)

  • increased grooming behavior(MP:0001441)

  • increased serotonin level(MP:0010069)

  • nervous system phenotype(MP:0003631)

  • postnatal growth retardation(MP:0001732)

  • postnatal lethality, incomplete penetrance(MP:0011086)

  • skin lesions(MP:0001212)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, bovine tau cDNA, mouse Olfr151 genomic DNA, mouse Pcdha genomic DNA, mouse Cnga2 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior consent on use of it from Professor Peter Monbaerts, The Rockefeller University. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate the treatment of such invention. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. The RECIPIENT, which wishes to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research, is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorTakeshi Yagi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data