Strain Data Sheet

RBRC02109

Strain Information

Image
BRC No.RBRC02109
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Gmcl1<tm1Nkn>
Former Common namemouse germ cell-less homologue-1 KO, B6;129-Gmcl1<tm1Nak>
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Mouse germ cell-less as an essential component for nuclear integrity.
Kimura T, Ito C, Watanabe S, Takahashi T, Ikawa M, Yomogida K, Fujita Y, Ikeuchi M, Asada N, Matsumiya K, Okuyama A, Okabe M, Toshimori K, Nakano T
Mol. Cell. Biol., 23, 1304-1315 (2003). 12556490

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gmcl1germ cell-less, spermatogenesis associated 16Gmcl1targeted mutation 1, Toru Nakano
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal acrosome morphology(MP:0008898)

  • abnormal cell nucleus morphology(MP:0003111)

  • abnormal sperm flagellum morphology(MP:0008892)

  • abnormal sperm head morphology(MP:0009230)

  • abnormal sperm nucleus morphology(MP:0009232)
    • more 12 phenotypes
  • abnormal spermatocyte morphology(MP:0006379)

  • abnormal spermatogenesis(MP:0001156)

  • abnormal spermiation(MP:0004182)

  • abnormal spermiogenesis(MP:0001932)

  • absent sperm flagellum(MP:0008545)

  • asthenozoospermia(MP:0002675)

  • decreased litter size(MP:0001935)

  • globozoospermia(MP:0002686)

  • multinucleated giant male germ cells(MP:0020550)

  • oligozoospermia(MP:0002687)

  • reduced male fertility(MP:0001922)

  • teratozoospermia(MP:0005578)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPGK promoter, ネオマイシン耐性遺伝子, PGK poly A signal
    Research applicationReproductive Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 23, 1304-1315 ( 2003).The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
    DepositorToru Nakano (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data