Strain Data Sheet

RBRC02105

Strain Information

Image
BRC No.RBRC02105
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Spen<tm2.1Hon>/HonRbrc
Former Common nameMint floxed mouse, B6.Cg-Spen<tm2.1>/HonRbrc
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Drs. Diasuke Yabe and Tasuku Honjo at Kyoto University in 2004.
Strain descriptionB6.Cg-Spen<tm2.1>. Mint, Msx2 interacting nuclear target protein which is a suppressor factor of a Notch/RBP-J signaling pathway, plays a role in fate determination. This strain has a loxP-flanked Mint allele. Conditional Mint deficient mice can be generated by crossing with tissue-specific Cre mice. This strain is useful for elucidating the mechanism of Notch/RBP-J signaling pathway in mammals.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
References
Generation of a conditional knockout allele for mammalian Spen protein Mint/SHARP.
Yabe D, Fukuda H, Aoki M, Yamada S, Takebayashi S, Shinkura R, Yamamoto N, Honjo T
Genesis 45(5):300-306 (2007). 17457934

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Spenspen family transcription repressor4Spentargeted mutation 2.1, Tasuku Honjo
loxPphage P1 loxP4loxP
loxPphage P1 loxP4loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal brain size(MP:0000771)

  • abnormal telencephalon morphology(MP:0000787)

  • decreased brain weight(MP:0002175)

  • decreased follicular B cell number(MP:0008174)

  • decreased marginal zone B cell number(MP:0008182)
    • more 7 phenotypes
  • enlarged lateral ventricles(MP:0008535)

  • increased follicular B cell number(MP:0008173)

  • increased marginal zone B cell number(MP:0008181)

  • postnatal growth retardation(MP:0001732)

  • premature death(MP:0002083)

  • small hippocampus(MP:0008283)

  • thin cerebral cortex(MP:0006254)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse Spen genomic DNA
    Research applicationCre/loxP system
    Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Yabe et al., Genesis 45(5):300-306 (2007).
    3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data