Strain Data Sheet

RBRC02103

Strain Information

Image
BRC No.RBRC02103
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Spen<tm2.1Hon>/HonRbrc
Former Common nameMint floxed mouse (C57BL/6 backgroud), B6.Cg-Spen<tm2.1>/HonRbrc
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Diasuke Yabe and Tasuku Honjo, Kyoto University in 2004. C57BL/6 background.
Strain descriptionMint, Msx2 interacting nuclear target protein which is a suppressor factor of a Notch/RBP-J signaling pathway, plays a role in fate determination. This strain has a loxP-flanked Mint allele. Conditional Mint deficient mice can be generated by crossing with tissue-specific Cre mice. This strain is useful for elucidating the mechanism of Notch/RBP-J signaling pathway in mammals.
Colony maintenanceSibling Mating (Homozygote x Homozygote)Homozygous mutant mice are viable and fertile.
References
Generation of a conditional knockout allele for mammalian Spen protein Mint/SHARP.
Yabe D, Fukuda H, Aoki M, Yamada S, Takebayashi S, Shinkura R, Yamamoto N, Honjo T
Genesis 45(5):300-306 (2007). 17457934

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Spenspen family transcription repressor4Spentargeted mutation 2.1, Tasuku Honjo
loxPphage P1 loxP4loxP
loxPphage P1 loxP4loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse Spen genomic DNA
    Research applicationCre/loxP system
    Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Yabe et al., Genesis 45(5):300-306 (2007).
    3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data