RBRC02102

Information1

Image
BRC No.RBRC02102
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Arpc3<tm1Jtak>/JtakRbrc
Former Common nameArpc3-KO
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Junji Takeda at Osaka University in 2005. A PGK-neo selection cassette replaced a fragment of exon 2 of Arpc3 gene.
Strain descriptionArpc3 is a component of the Arp2/3 complex, which plays a major role in actin nucleation, filament binding, and Y-shaped branching to reorganize filamentous actin in response to extrinsic or intrinsic signaling for cell motility.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6J)
ReferencesMol. Cell. Biol., 26, 6185-6196 (2006). 16880528

Health Report

Examination Date / Room / Rack2008/03/17Room:4-ARack:K
2008/01/14Room:4-ARack:K
2007/11/12Room:4-ARack:K
2007/09/18Room:4-ARack:K
2007/07/17Room:4-ARack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Arpc3actin related protein 2/3 complex, subunit 35Arpc3<tm1Jtak>targeted mutation 1, Junji Takeda

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5

Information2

Donor DNAmouse Arpc3 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationCell Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Molecular and Cellular Biology, 26, 6185-6196 (2006).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorJunji Takeda (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Takeda Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data