RBRC02101

Information1

Image
BRC No.RBRC02101
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameIAP-Q14gfp
Former Common nameIAP-Q14gfp
H-2 Haplotype
ES Cell line
Background strain
Appearanceagouti [A/A B/B C/C]
Strain developmentDeveloped by Drs. Junji Takeda and Kyoji Horie at Osaka University in 2004.
Strain descriptionC3H/HeN-Tg(IAP-Q14GFP)1Khor. These transgenic mice express GFP in response to intracisternal A particle (IAP) elements that are long terminal repeat (LTR)-type mouse retrotransposons consisting of full-length and internally deleted types. This strain is useful for a reporter for retrotansposition.
Colony maintenanceBackcross to C3H/HeNCrlCrlj
References

Health Report

Examination Date / Room / Rack2009/05/19Room:4-BRack:E
2009/05/18Room:4-BRack:E
2009/03/16Room:4-BRack:E
2009/01/19Room:4-BRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), human gamma-globin intron

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptorUNSA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SDsplice donor (SD)UNSD

Information2

Donor DNAmouse intracisternal A proticle element, Jellyfish GFP cDNA, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), human gamma-globin intron, SA (splice donor), SD (splice acceptor)
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorJunji Takeda (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Takeda Lab HP

BRC mice in Publications

No Data