Strain Data Sheet

RBRC02101

Strain Information

Image
BRC No.RBRC02101
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC3H/HeN-Tg(IAP-Q14GFP)1Khor/KhorRbrc
Former Common nameIAP-Q14gfp
H-2 Haplotype
ES Cell line
Background strain
Appearanceagouti [A/A B/B C/C]
Strain developmentDeveloped by Drs. Junji Takeda and Kyoji Horie at Osaka University in 2004.
Strain descriptionC3H/HeN-Tg(IAP-Q14GFP)1Khor. These transgenic mice express GFP in response to intracisternal A particle (IAP) elements that are long terminal repeat (LTR)-type mouse retrotransposons consisting of full-length and internally deleted types. This strain is useful for a reporter for retrotansposition.
Colony maintenanceBackcross to C3H/HeNCrlCrlj
References
Translation from nonautonomous type IAP retrotransposon is a critical determinant of transposition activity: implication for retrotransposon-mediated genome evolution.
Ei-Suke Saito, Vincent W Keng, Junji Takeda, Kyoji Horie
Genome Res., 18(6):859-68 (2008). 18456863

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2rabbit beta-globin polyAUNHBB2
HBG2human gamma-globinUNHBG2
IAPintracisternal A proticle elementUNIAPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
hrGFPhumanized Renilla green fluorescent proteinUNhrGFPhuman EEF1A1 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), IAP-LTR, mouse intracisternal A proticle element, human EEF1A1 promoter, Jellyfish hrGFP cDNA, human gamma-globin intron, SA (splice donor), SD (splice acceptor), rabbit HBB2 polyA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data