Strain Data Sheet

RBRC02099

Strain Information

Image
BRC No.RBRC02099
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Plxna4<tm1Hfu>/HfuRbrc
Former Common namePlexin-A4 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Sudo and Fujisawa, Nagoya University in 2001. Mutant mice were backcrossed to C57BL/6Cr for 14 times.
Strain descriptionB6.Cg-Plxna4<tm1Hfu>. Plexin-A4 serves as a direct receptor for two class 6 transmembrane semaphorins, Sema6A and Sema6B, both of which act as repellents for sympathetic ganglion (SG) axons. Plexin-A4 homozygous mutant mice show defective in the trajectory and projection of peripheral sensory axons and GS axons and the formation of the anterior commissure and the barrels.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)
References
Plexin-a4 mediates axon-repulsive activities of both secreted and transmembrane semaphorins and plays roles in nerve fiber guidance.
Suto F, Ito K, Uemura M, Shimizu M, Shinkawa Y, Sanbo M, Shinoda T, Tsuboi M, Takashima S, Yagi T, Fujisawa H
J. Neurosci., 25, 3628-3637 (2005). 15814794

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRESinternal ribosomal entry site (EMCV)6
Plxna4plexin A46Plxna4targeted mutation 1, Hajime Fujisawa
TakE. coli Tet repressor DNA binding protein (TetR), HSV VP16 transactivating domain cDNA6Tak
loxPphage P1 loxP6loxP
loxPphage P1 loxP6loxP
loxPphage P1 loxP6loxP
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal anterior commissure morphology(MP:0008225)

  • abnormal anterior commissure pars anterior morphology(MP:0010859)

  • abnormal anterior commissure pars posterior morphology(MP:0010860)

  • abnormal axon guidance(MP:0002961)

  • abnormal cranial nerve morphology(MP:0001056)
    • more 10 phenotypes
  • abnormal facial nerve morphology(MP:0001071)

  • abnormal glossopharyngeal nerve morphology(MP:0001073)

  • abnormal hippocampal mossy fiber morphology(MP:0002761)

  • abnormal neuron differentiation(MP:0009937)

  • abnormal sensory neuron innervation pattern(MP:0000968)

  • abnormal spinal nerve morphology(MP:0001077)

  • abnormal sympathetic postganglionic fiber morphology(MP:0008312)

  • abnormal trigeminal nerve morphology(MP:0001065)

  • abnormal vagus nerve morphology(MP:0001074)

  • disorganized barrel cortex(MP:0000861)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, Encephalomyocarditis virus IRES, Tak cDNA(E. coli Tet repressor DNA binding protein (TetR), HSV VP16 transactivating domain cDNA), mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, polyA, mouse plexin-A4 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    Neurobiology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 25, 3628-3637 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr.
    Fumikazu Suto, National Institute of Neuroscience, National Center of Neurology and Psychiatry, E-mail: fsuto@ncnp.go.jp) to transfer the BIOLOGICAL RESOURCE.
    DepositorHajime Fujisawa (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data