RBRC02098

Information1

Image
BRC No.RBRC02098
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Plxna2<tm1Hfu>/HfuRbrc
Former Common namePlexin-A2 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NCrSlc
Appearance
Strain development
Strain description
Colony maintenance
ReferencesNeuron, 53, 535-547 (2007) 17296555

Health Report

Examination Date / Room / Rack2008/02/18Room:3-CRack:C
2007/12/17Room:3-CRack:C
2007/10/15Room:3-CRack:C
2007/08/20Room:3-CRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Plxna2plexin A21Plxna2<tm1Hfu>targeted mutation 1, Hajime Fujisawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
TakE. coli Tet repressor DNA binding protein (TetR), HSV VP16 transactivating domain cDNA1Tak

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, Encephalomyocarditis virus IRES, Tak (E. coli Tet repressor DNA binding protein (TetR), HSV VP16 transactivating domain cDNA), mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, polyA, mouse plexin-A2 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
Neurobiology Research
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Suto et al., Neuron, 53(4):535-547 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr.
Fumikazu Suto, National Institute of Neuroscience, National Center of Neurology and Psychiatry, E-mail: fsuto@ncnp.go.jp) to transfer the BIOLOGICAL RESOURCE.
DepositorHajime Fujisawa (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data