BRC No.RBRC02097
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Izumo1<tm1Osb>
Former Common nameIzumo KO
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Drs. Naokazu Inoue and Masaru Okabe at Osaka University in 2003. 129, C57BL/6 and DBA/2 mixed background.
Strain descriptionIzumo1 is a immunoglobulin super family protein that is required for sperm to fuse with eggs. Homozygous mutant mice are viable but males are sterile. The Izumo-deficient sperm can bind to an egg, and even burrow through the zona pellucida, but are incapable of fusing with egg.
Colony maintenanceSibling Mating (Hemizygote x Wild-type, Hemizygote) Homozygous mutant males are sterile.
ReferencesNature, 434, 234-238 (2005). 15759005

Health Report

Examination Date / Room / Rack2009/03/09Room:2-検Rack:D


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Izumo1izumo sperm-egg fusion 17Izumo1<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse Izumo genomic DNA, mouse PGK promoter, E. coli neo
Research applicationReproductive Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 434, 234-238 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data