RBRC02096

Information1

Image
BRC No.RBRC02096
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-floxed Neo-EGFP)REP08Osb
Former Common nameREP-08
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masato Ikawa, Faculty of Pharmaceutical Sciences, Osaka University in 2004. The transgene was injected into the D3 ES cells. The mutant mice were crossed to C57BL/6NCrSlc.
Strain descriptionCAG-GFP conditional transgenic mice (REP08 mice). The transgene consists of the CAG promoter and the EGFP cDNA separated by a floxed neo cassette. When the floxed neo cassette is excised by crossing with Cre mice, EGFP is expressed. The transgene was injected into D3 ES cells to generate mice having a single copy of the transgene. The level of GFP fluorescence is relatively low (Developer, personal communication).
Colony maintenanceCarrier x Carrier [C57BL/6NCrSlc]
ReferencesBiochem. Biophys. Res. Commun., 321, 275-279 (2004). 15358172

Health Report

Examination Date / Room / Rack2018/10/15Room:3-CRack:J
2018/08/20Room:3-CRack:J
2018/06/18Room:3-CRack:J
2018/04/16Room:3-CRack:J
2018/02/19Room:3-CRack:J
2017/12/18Room:3-CRack:J
2017/10/16Room:3-CRack:J
2017/08/21Room:3-CRack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, E. coli neo, pA, Jellyfish GFP cDNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
General Purpose
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem Biophys Res Commun., 321, 275-279 (2004).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

Miyoshi K, Yanagi S, Kawahara K, Nishio M, Tsubouchi H, Imazu Y, Koshida R, Matsumoto N, Taguchi A, Yamashita S, Suzuki A, Nakazato M.
Epithelial pten controls acute lung injury and fibrosis by regulating alveolar epithelial cell integrity.
Am. J. Respir. Crit. Care Med. 187(3) 262-75(2013) 23239155

Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald JA, Kimata K, Taniguchi S, Itano N.
Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization.
Cancer Res. 70(18) 7073-83(2010) 20823158

Maeda K, Kobayashi Y, Udagawa N, Uehara S, Ishihara A, Mizoguchi T, Kikuchi Y, Takada I, Kato S, Kani S, Nishita M, Marumo K, Martin TJ, Minami Y, Takahashi N.
Wnt5a-Ror2 signaling between osteoblast-lineage cells and osteoclast precursors enhances osteoclastogenesis.
Nat. Med. 18(3) 405-12(2012) 22344299