Strain Data Sheet

RBRC02096

Strain Information

Image
BRC No.RBRC02096
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-floxed Neo-EGFP)REP08Osb
Former Common nameREP-08
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masato Ikawa, Faculty of Pharmaceutical Sciences, Osaka University in 2004. The transgene was injected into the D3 ES cells. The mutant mice were crossed to C57BL/6NCrSlc.
Strain descriptionCAG-GFP conditional transgenic mice (REP08 mice). The transgene consists of the CAG promoter and the EGFP cDNA separated by a floxed neo cassette. When the floxed neo cassette is excised by crossing with Cre mice, EGFP is expressed. The transgene was injected into D3 ES cells to generate mice having a single copy of the transgene. The level of GFP fluorescence is relatively low (Developer, personal communication).
Colony maintenanceCarrier x Carrier [C57BL/6NCrSlc]
References
Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
Matsumura H, Hasuwa H, Inoue N, Ikawa M, Okabe M
Biochem. Biophys. Res. Commun., 321, 275-279 (2004). 15358172

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
loxPphage P1 loxPUNloxP
loxPphage P1 loxPUNloxP
neoneomycin resistance gene (E. coli)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, E. coli neo, pA, Jellyfish GFP cDNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    General Purpose
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem Biophys Res Commun., 321, 275-279 (2004).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Miyoshi K, Yanagi S, Kawahara K, Nishio M, Tsubouchi H, Imazu Y, Koshida R, Matsumoto N, Taguchi A, Yamashita S, Suzuki A, Nakazato M.
    Epithelial Pten controls acute lung injury and fibrosis by regulating alveolar epithelial cell integrity.
    Am J Respir Crit Care Med 187(3) 262-75(2013) 23239155
    Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald JA, Kimata K, Taniguchi S, Itano N.
    Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization.
    Cancer Res 70(18) 7073-83(2010) 20823158
    Maeda K, Kobayashi Y, Udagawa N, Uehara S, Ishihara A, Mizoguchi T, Kikuchi Y, Takada I, Kato S, Kani S, Nishita M, Marumo K, Martin TJ, Minami Y, Takahashi N.
    Wnt5a-Ror2 signaling between osteoblast-lineage cells and osteoclast precursors enhances osteoclastogenesis.
    Nat Med 18(3) 405-12(2012) 22344299
    Tamaki T, Uchiyama Y, Okada Y, Tono K, Masuda M, Nitta M, Hoshi A, Akatsuka A.
    Clonal differentiation of skeletal muscle-derived CD34(-)/45(-) stem cells into cardiomyocytes in vivo.
    Stem Cells Dev 19(4) 503-12(2010) 19634996