BRC No.RBRC02095
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Th-EGFP)21-31Koba
Former Common nameB6.Cg-Tg(TH-GFP)21-31
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162.
Strain descriptionThe TH-GFP transgenic mouse was generated to express GFP in the majority of midbrain dopamine neurons under the control of the rat TH gene promoter. Dopamine is a neurotransmitter which mediates motor control, cognition, emotion, reward, and neuroendocrine functions. Dysfunction of dopamine-producing neurons in the midbrain is involved in some neurological and neuropsychiatric diseases such as Parkinson’s disease and schizophrenia. These dopamine neurons express tyrosine hydroxylase (TH), a key enzyme of catecholamine biosynthesis. This transgenic mouse is useful for visualizing dopamine neurons to study the physiology and pathogenesis of dopamine neurons. Homozygous transgenic mice are lethal.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesJ. Neurochem., 82, 295-304 (2002). 12124430

Health Report

Examination Date / Room / Rack2020/10/05Room:4-ARack:K


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Jellyfish)UNrat tyrosine hydroxylase (TH) promoter


Donor DNArat tyrosine hydroxylase (TH) promoter, rabbit beta-globin intron, jelly fish green fluorescent protein (GFP) gene, rabbit beta-globin poly A, SV40 early gene poly A
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurochem., 82, 295-304 (2002).
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)
Construct map
Transgene sequence
Mouse of the Month May 2008
Genetic Background

BRC mice in Publications

Masi A,, Narducci R, Resta F, Carbone C, Kobayashi K, Mannaioni G.
Differential contribution of Ih to the integration of excitatory synaptic inputs in Substantia Nigra pars compacta and Ventral Tegmental Area dopaminergic neurons.
Eur. J. Neurosci. (2015) 26354486

Kiyokage E, Kobayashi K, Toida K.
Spatial distribution of synapses on tyrosine hydroxylase expressing juxtaglomerular cells in the mouse olfactory glomerulus.
J. Comp. Neurol. (2016) 27864931

Suzuki Y, Kiyokage E, Sohn J, Hioki H, Toida K.
Structural basis for serotonergic regulation of neural circuits in the mouse olfactory bulb.
J. Comp. Neurol. (2014) 25234191

Sawada M, Kaneko N, Inada H, Wake H, Kato Y, Yanagawa Y, Kobayashi K, Nemoto T, Nabekura J, Sawamoto K.
Sensory Input Regulates Spatial and Subtype-Specific Patterns of Neuronal Turnover in the Adult Olfactory Bulb.
J. Neurosci. 31(32) 11587-11596(2011) 21832189

Zhu S, Zhao C, Wu Y, Yang Q, Shao A, Wang T, Wu J, Yin Y, Li Y, Hou J, Zhang X, Zhou G, Gu X, Wang X, Bustelo XR, Zhou J.
Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking.
Nat. Neurosci. (2015) 26147533