Strain Data Sheet

RBRC02095

Information1

Image
BRC No.RBRC02095
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Th-EGFP)21-31Koba
Former Common nameB6.Cg-Tg(TH-GFP)21-31
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162.
Strain descriptionThe TH-GFP transgenic mouse was generated to express GFP in the majority of midbrain dopamine neurons under the control of the rat TH gene promoter. Dopamine is a neurotransmitter which mediates motor control, cognition, emotion, reward, and neuroendocrine functions. Dysfunction of dopamine-producing neurons in the midbrain is involved in some neurological and neuropsychiatric diseases such as Parkinson’s disease and schizophrenia. These dopamine neurons express tyrosine hydroxylase (TH), a key enzyme of catecholamine biosynthesis. This transgenic mouse is useful for visualizing dopamine neurons to study the physiology and pathogenesis of dopamine neurons. Homozygous transgenic mice are lethal.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesJ. Neurochem., 82, 295-304 (2002). 12124430

Health Report

Examination Date / Room / Rack2022/05/23Room:4-ARack:K
2022/03/07Room:4-ARack:K
2021/12/06Room:4-ARack:K
2021/09/08Room:4-ARack:K
2021/06/07Room:4-ARack:K
2021/03/22Room:4-ARack:K
2021/03/15Room:4-ARack:K
2021/03/08Room:4-ARack:K
2020/12/14Room:4-ARack:K
2020/10/05Room:4-ARack:K
2020/07/06Room:4-ARack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNrat tyrosine hydroxylase (TH) promoter

Information2

Donor DNArat tyrosine hydroxylase (TH) promoter, rabbit beta-globin intron, jelly fish green fluorescent protein (GFP) gene, rabbit beta-globin poly A, SV40 early gene poly A
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurochem., 82, 295-304 (2002).
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Construct map
Transgene sequence
Mouse of the Month May 2008
Genetic Background
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Heo JY, Nam MH, Yoon HH, Kim J, Hwang YJ, Won W, Woo DH, Lee JA, Park HJ, Jo S, Lee MJ, Kim S, Shim JE, Jang DP, Kim KI, Huh SH, Jeong JY, Kowall NW, Lee J, Im H, Park JH, Jang BK, Park KD, Lee HJ, Shin H, Cho IJ, Hwang EM, Kim Y, Kim HY, Oh SJ, Lee SE, Paek SH, Yoon JH, Jin BK, Kweon GR, Shim I, Hwang O, Ryu H, Jeon SR, Lee CJ.
Aberrant Tonic Inhibition of Dopaminergic Neuronal Activity Causes Motor Symptoms in Animal Models of Parkinson's Disease.
Curr Biol 30(2) 276-291.e9(2020) 31928877

Masi A, Narducci R, Resta F, Carbone C, Kobayashi K, Mannaioni G.
Differential contribution of Ih to the integration of excitatory synaptic inputs in substantia nigra pars compacta and ventral tegmental area dopaminergic neurons.
Eur J Neurosci 42(9) 2699-706(2015) 26354486

Kiyokage E, Kobayashi K, Toida K.
Spatial distribution of synapses on tyrosine hydroxylase-expressing juxtaglomerular cells in the mouse olfactory glomerulus.
J Comp Neurol 525(5) 1059-1074(2017) 27864931

Suzuki Y, Kiyokage E, Sohn J, Hioki H, Toida K.
Structural basis for serotonergic regulation of neural circuits in the mouse olfactory bulb.
J Comp Neurol 523(2) 262-80(2015) 25234191

Sawada M, Kaneko N, Inada H, Wake H, Kato Y, Yanagawa Y, Kobayashi K, Nemoto T, Nabekura J, Sawamoto K.
Sensory input regulates spatial and subtype-specific patterns of neuronal turnover in the adult olfactory bulb.
J Neurosci 31(32) 11587-96(2011) 21832189

Zhu S, Zhao C, Wu Y, Yang Q, Shao A, Wang T, Wu J, Yin Y, Li Y, Hou J, Zhang X, Zhou G, Gu X, Wang X, Bustelo XR, Zhou J.
Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking.
Nat Neurosci 18(8) 1084-93(2015) 26147533

Kosaka T, Pignatelli A, Kosaka K.
Heterogeneity of tyrosine hydroxylase expressing neurons in the main olfactory bulb of the mouse.
Neurosci Res 157 15-33(2020) 31629793