Strain Data Sheet

RBRC02091

Strain Information

Image
BRC No.RBRC02091
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Pcdha<tm5.1Tyag> Olfr151<tm25Mom>/TyagRbrc
Former Common nameM71-LacZ/dA
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University. The mutant mice were generated by crossing with CNR delta A (RBRC01691) (Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University) and M71-lacZ (Peter Monbaerts, The Rockefeller University) mice.
Strain descriptionCNR (Pcdha) delta A mutant and M71 (Olfr151) gene ires-taulacZ knock-in double mutant mice.
Colony maintenancePcdha: Homozygote x Homozygote; Olfr151: Homozygote x Homozygote
References
The protocadherin-alpha family is involved in axonal coalescence of olfactory sensory neurons into glomeruli of the olfactory bulb in mouse.
Hasegawa S, Hamada S, Kumode Y, Esumi S, Katori S, Fukuda E, Uchiyama Y, Hirabayashi T, Mombaerts P, Yagi T
Mol Cell Neurosci, 38, 66-79 (2008). 18353676

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pcdhaprotocadherin alpha cluster18Pcdhatargeted mutation 5.1, Takeshi Yagi
loxPphage P1 loxP18loxP
IRESinternal ribosomal entry site (EMCV)9
Olfr151olfactory receptor 1519Olfr151targeted mutation 25, Peter Mombaerts
lacZbeta-galactosidase (E. coli)9
loxPphage P1 loxP9loxP
taubovine tau protein cDNA9tau

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal dentate gyrus morphology(MP:0000812)

  • abnormal hippocampus morphology(MP:0000807)

  • abnormal serotonergic neuron morphology(MP:0003877)

  • abnormal serotonin level(MP:0005322)

  • decreased body weight(MP:0001262)
    • more 2 phenotypes
  • increased grooming behavior(MP:0001441)

  • skin lesions(MP:0001212)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP, E. coli lacZ, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires),bovine tau cDNA, mouse Olfr151 genomic DNA, mouse Pcdha genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior consent on use of it from Professor Peter Monbaerts, The Rockefeller University. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate the treatment of such invention. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. The RECIPIENT, which wishes to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research, is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorTakeshi Yagi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese), Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data