Strain Data Sheet

RBRC02087

Strain Information

Image
BRC No.RBRC02087
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Pcdha<tm(AdAB)> Olfr17<tm1Mom>/TyagRbrc
Former Common nameP2-LacZ/AdAB
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University. The mutant mice were generated by crossing with CNR AdAB (RBRC01687) (Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University) and P2-lacZ (Peter Monbaerts, The Rockefeller University) mice.
Strain descriptionCNR (Pcdha) AdAB mutant (CR1, CR2 and CR3a were deleted) and P2 (Olfr17) gene ires-taulacZ knock-in double mutant mice.
Colony maintenancePcdha: Homozygote x Homozygote; Olfr17: Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
References
The protocadherin-alpha family is involved in axonal coalescence of olfactory sensory neurons into glomeruli of the olfactory bulb in mouse.
Hasegawa S, Hamada S, Kumode Y, Esumi S, Katori S, Fukuda E, Uchiyama Y, Hirabayashi T, Mombaerts P, Yagi T
Mol Cell Neurosci, 38, 66-79 (2008). 18353676

Visualizing an olfactory sensory map.
Mombaerts P, Wang F, Dulac C, Chao S K, Nemes A, Mendelsohn M, Edmondson J, Axel R
Cell, 87, 675-686 (1996). 8929536

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pcdhaprotocadherin alpha cluster18Pcdhatargeted mutation
loxPphage P1 loxP18loxP
IRESinternal ribosomal entry site (EMCV)7
Olfr17olfactory receptor 177Olfr17targeted mutation 1, Peter Mombaerts
lacZbeta-galactosidase (E. coli)7
loxPphage P1 loxP7loxP
taubovine tau protein cDNA7tau

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal olfactory neuron innervation pattern(MP:0012014)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), bovine tau cDNA, E.coli lacZ, mouse Pcdha genomic DNA, mouse Olfr17 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior consent on use of it from Professor Peter Monbaerts, The Rockefeller University. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate the treatment of such invention. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. The RECIPIENT, which wishes to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research, is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorTakeshi Yagi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data