RBRC02080

Information1

Image
BRC No.RBRC02080
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pcdha<tm6Tyag>/TyagRbrc
Former Common nameG16, CNR G16
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2005. TT2 ES cells were used.
Strain descriptionCNR (Pcdha) G16Neo mice. A floxed PGK-neo cassette was inserted between exons v1 and v2. Homozygous mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing with C57BL/6J(Crlj)]
ReferencesJ Biol Chem., 284, 32002-32014 (2009). 19797050
Front Mol Neurosci., 5, 1-14 (2012). 23087612

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pcdhaprotocadherin alpha cluster18Pcdha<tm6Tyag>targeted mutation 6, Takeshi Yagi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationCell Biology Research
Cre/loxP system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)

BRC mice in Publications

No Data