RBRC02078

Information1

Image
BRC No.RBRC02078
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Slp2<tm1Mitf>
Former Common nameSlp2-a KO mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Chika Saegusa and Mitsunori Fukuda, RIKEN Brain Science Institute in 2006. E14 ES cells derived from 129P2/OlaHsd were used to generated the knockout mice. The mutant mice were crossed C57BL/6.
Strain descriptionSlp2 gene knockout mice. Exon 1 of the Slp2 was replaced with a neo cassette. Slp (Synaptoagmin-like protein) is a Rab family of low-molecular-G protein, and known as a molecule implicated in the Rab27-dependent membrane transport. Homozygous mutant mice shows an abnormal gastric surface mucus cell morphology and reduced number of mucus granules.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesGenes Cells, 11, 623-631 (2006). 16716193

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sytl2synaptotagmin-like 27Sytl2<tm1Mitf>targeted mutation 1, Mitsunori Fukuda

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli neo, mouse PGK promoter, mouse Slp2-a genomic DNA
Research applicationCell Biology Research
Dermatology Research
Internal/Organ Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Saegusa, C., Itohara S. and Fukuda. M.Role of Rab27B-binding protein Slp1 in pancreatic acinar cells. In preparationIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Permission of Dr. Mitsunori Fukuda is requried before use of the Slp1-KO mice.
DepositorMitsunori Fukuda (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data