Strain Data Sheet

RBRC02077

Strain Information

Image
BRC No.RBRC02077
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Sytl1<tm1Mitf>/MitfRbrc
Former Common nameSlp1 KO mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Chika Segusa and Mitsunori Fukuda at RIKEN BSI. A neomysin resistance cassette were inserted into the exon 1 of the Slp1 gene. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionB6;129P2-Sytl1<tm1Mitf>. Slp (Synaptoagmin-like protein) is a Rab family of low-molecular-G protein, and known as a molecule implicated in the Rab27-dependent membrane transport. Homozygous mutant mice shows an increased number of zymogen granules in the pancreatic acinar cells.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References
Expression of Rab27B-binding protein Slp1 in pancreatic acinar cells and its involvement in amylase secretion.
Saegusa C, Kanno E, Itohara S, Fukuda M
Arch Biochem Biophys. 2008 Jul 1;475(1):87-92. 18477466

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sytl1synaptotagmin-like 14Sytl1targeted mutation 1, Mitsunori Fukuda
neoneomycin resistance gene (E. coli)4

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal pancreas secretion(MP:0002694)

  • abnormal pancreatic amylase secretion(MP:0014096)

  • pancreatic acinar cell zymogen granule accumulation(MP:0009161)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Slp1 genomic DNA, E. coli neo, mouse PGK promoter
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Saegusa, C., Kanno E., Itohara S. and Fukuda. M. Expression of Rab27B-binding protein Slp1 in pancreatic acinar cells and its involvement in amylase secretion. Arch Biochem Biophys. 2008 Jul 1;475(1):87-92.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Permission of Dr. Mitsunori Fukuda is requried before use of the Slp1-KO mice.
    DepositorMitsunori Fukuda (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data