Strain Data Sheet

RBRC02032

Strain Information

Image
BRC No.RBRC02032
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C3-Tg(CAG/Acr-EGFP)CX-FM139Osb
Former Common nameB6C3F1-Tg(CAG/Acr-EGFP)CX-FM139Osb (Nov. 2011), B6D2F1-Tg(CAG-EGFP)CX-FM139Osb
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a b/b C/C]
Strain developmentDeveloped by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. This transgenic line was generated by coinjected with act-EGFP and acr-EGFP transgene constructs into fertilized eggs. The mice were crossed to C57BL/6.
Strain descriptionTransgenic mice expressing GFP ubiquitously from CAG-EGFP and in the sperm acrosome from acr3-EGFP. GFP expression is detected in germline cells at early stage (8 cells). Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG-EGFP, in which EGFP is connected to the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG). The transgene is located in chromosome X by FISH.
Colony maintenanceCarrier x Noncarrier [C57BL/6NJcl]
References
FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Nakanishi T, Kuroiwa A, Yamada S, Isotani A, Yamashita A, Tairaka A, Hayashi T, Takagi T, Ikawa M, Matsuda Y, Okabe M
Genomics, 80(6):564-574 (2002). 12504848

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)XCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACX-EGFP (Jellyfish GFP cDNA, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, rabbit beta-globin polyadenylation signal
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genomics, 80(6):564-574 (2002).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data