Strain Data Sheet

RBRC01969

Strain Information

Image
BRC No.RBRC01969
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(CAG-EGFP)1Yam Fbxo15<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Shinya Yamanaka at Nara Institute of Science and Technology in 2004. Mixed background. KO: Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exon 3 through 7. The mutant mice were backcross to C57BL/6J for several times. Tg: The EGFP under the CAG promoter was injected into the pronuclei of fertilized eggs. ICR background.
Strain descriptionFbxo15 knockout and CAG-EGFP transgenic double mutant mice. Fbxo15, F-box protein 15 is a novel target of undifferentiated-cell-specific transcripton factor Oct3/4 playing essential roles in self-renewal of ES cells and mouse early development. Fbxo15 is predominantly expressed in mouse undifferentiated ES cells. Inactivation of Oct3/4 in ES cells lead to rapid disappearance of Fbxo15 expression.
Colony maintenanceTg: Carrier x Noncarrier; KO: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development.
Yoshimi Tokuzawa, Eiko Kaiho, Masayoshi Maruyama, Kazutoshi Takahashi, Kaoru Mitsui, Mitsuyo Maeda, Hitoshi Niwa, Shinya Yamanaka
Mol. Cell. Biol., 23, 2699-2708 (2003). 12665572

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Kazutoshi Takahashi, Shinya Yamanaka
Cell, 126, 663-676 (2006). 16904174

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fbxo15
MGI:1354755		F-box protein 1518Fbxo15<tm1Yam>
MGI:2655776		targeted mutation 1, Shinya Yamanaka
IRES internal ribosomal entry site (EMCV)18
lacZ beta-galactosidase (E. coli)18
neo neomycin resistance gene (E. coli)18
SV40 polyA18
GFP Green Fluorescent Protein (Aequorea victoria)UN CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), green flourescent protein (jellyfish)、E. coli lacZ, E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 polyA, mouse Fbxo15 genomic DNA
    Research applicationDevelopmental Biology Research
    Specific Term and Conditions
    DepositorShinya Yamanaka (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: Japanese (Word) / English(Word)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data