RBRC01969

Information1

Image
BRC No.RBRC01969
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(CAG-EGFP)1Yam Fbxo15tm1Yam
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Shinya Yamanaka at Nara Institute of Science and Technology in 2004. Mixed background. KO: Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exon 3 through 7. The mutant mice were backcross to C57BL/6J for several times. Tg: The EGFP under the CAG promoter was injected into the pronuclei of fertilized eggs. ICR background.
Strain descriptionFbxo15 knockout and CAG-EGFP transgenic double mutant mice. Fbxo15, F-box protein 15 is a novel target of undifferentiated-cell-specific transcripton factor Oct3/4 playing essential roles in self-renewal of ES cells and mouse early development. Fbxo15 is predominantly expressed in mouse undifferentiated ES cells. Inactivation of Oct3/4 in ES cells lead to rapid disappearance of Fbxo15 expression.
Colony maintenanceTg: Carrier x Noncarrier; KO: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesCell, 126, 663-676 (2006). 16904174
Mol. Cell. Biol., 23, 2699-2708 (2003). 12665572

Health Report

Examination Date / Room / Rack2014/02/18Room:3-BRack:E
2013/12/16Room:3-BRack:E
2013/10/21Room:3-BRack:E
2013/08/26Room:3-BRack:E
2013/06/17Room:3-BRack:E
2013/04/15Room:3-BRack:E
2012/12/10Room:3-BRack:E
2012/10/15Room:3-BRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Fbxo15F-box protein 1518Fbxo15<tm1Yam>targeted mutation 1, Shinya Yamanaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), green flourescent protein (jellyfish)、E. coli lacZ, E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 polyA, mouse Fbxo15 genomic DNA
Research applicationDevelopmental Biology Research
Specific Term and Conditions
DepositorShinya Yamanaka (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.MATERIAL TRANSFER AGREEMENT (Japanese (Word) / English(Word)
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data