Strain Data Sheet

RBRC01967

Information1

Image
BRC No.RBRC01967
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Nanog<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exson 2 whcich contains the homeodomain. The mutant mice were backcross to C57BL/6J.
Strain descriptionNanog gene is a important factor of maintaining ES cell pluripotent differentiation.Homozygous mutant mice are embryonic lethal.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesCell, 113, 631-642 (2003). 12787504

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
NanogNanog homeobox6Nanog<tm1Yam>targeted mutation 1, Shinya Yamanaka2410002E02Rik, ecat4, ENK

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)6internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;

Information2

Donor DNAmouse Nanog genomic DNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 poly A
Research applicationDevelopmental Biology Research
Specific Term and Conditions
DepositorShinya Yamanaka (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: Japanese (Word) / English(Word)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data