RBRC01967

Information1

Image
BRC No.RBRC01967
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Nanog<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exson 2 whcich contains the homeodomain. The mutant mice were backcross to C57BL/6J.
Strain descriptionNanog gene is a important factor of maintaining ES cell pluripotent differentiation.Homozygous mutant mice are embryonic lethal.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesCell, 113, 631-642 (2003). 12787504

Health Report

Examination Date / Room / Rack2007/07/17Room:4-ARack:K
2007/05/22Room:4-ARack:K
2007/05/21Room:4-ARack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
NanogNanog homeobox6Nanog<tm1Yam>targeted mutation 1, Shinya Yamanaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)6

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6

Information2

Donor DNAmouse Nanog genomic DNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 poly A
Research applicationDevelopmental Biology Research
Specific Term and Conditions
DepositorShinya Yamanaka (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Genotyping protocol -PCR-
MATERIAL TRANSFER AGREEMENT (Japanese (Word) / English(Word)

BRC mice in Publications

No Data