Strain Data Sheet

RBRC01966

Information1

Image
BRC No.RBRC01966
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-ERas<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the ERas coding region. The mutant mice were backcross to C57BL/6J for 4 times.
Strain descriptionERas gene is important in the tumor-like grouwth properties of ES cells.Homozygous mutant mice develop normally and are fertile.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
ReferencesNature, 423, 541-545 (2003). 12774123

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ErasES cell-expressed RasXEras<tm1Yam>targeted mutation 1, Shinya Yamanakaecat5, Ha-Ras2, HRAS2, HRasp

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)Xinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)X

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)Xneo; neomycin;

Information2

Donor DNAE. coli lacZ, E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 pA
Research application
Specific Term and Conditions
DepositorShinya Yamanaka (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: Japanese (Word) / English(Word)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data