RBRC01966

Information1

Image
BRC No.RBRC01966
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-ERas<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the ERas coding region. The mutant mice were backcross to C57BL/6J for 4 times.
Strain descriptionERas gene is important in the tumor-like grouwth properties of ES cells.Homozygous mutant mice develop normally and are fertile.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
ReferencesNature, 423, 541-545 (2003). 12774123

Health Report

Examination Date / Room / Rack2009/02/09Room:3-5Rack:C
2008/12/08Room:3-5Rack:C
2008/10/06Room:3-5Rack:C
2008/08/04Room:3-5Rack:C
2008/06/09Room:3-5Rack:C
2008/02/04Room:3-5Rack:C
2007/12/03Room:3-5Rack:C
2007/10/01Room:3-5Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
ErasES cell-expressed RasXEras<tm1Yam>targeted mutation 1, Shinya Yamanaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)X

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)X

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)X

Information2

Donor DNAE. coli lacZ, E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 pA
Research application
Specific Term and Conditions
DepositorShinya Yamanaka (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
MATERIAL TRANSFER AGREEMENT (Japanese (Word) / English(Word)

BRC mice in Publications

No Data