Strain Data Sheet

RBRC01966

Strain Information

Image
BRC No.RBRC01966
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-ERas<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the ERas coding region. The mutant mice were backcross to C57BL/6J for 4 times.
Strain descriptionERas gene is important in the tumor-like grouwth properties of ES cells.Homozygous mutant mice develop normally and are fertile.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
References
Role of ERas in promoting tumour-like properties in mouse embryonic stem cells.
Kazutoshi Takahashi, Kaoru Mitsui, Shinya Yamanaka
Nature, 423, 541-545 (2003). 12774123

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Eras
MGI:2665023		ES cell-expressed RasXEras<tm1Yam> targeted mutation 1, Shinya Yamanaka
IRES internal ribosomal entry site (EMCV)X
lacZ beta-galactosidase (E. coli)X
neo neomycin resistance gene (E. coli)X
SV40 polyAX

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 pA, mouse ERas genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and Conditions
    DepositorShinya Yamanaka (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: Japanese (Word) / English(Word)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data