Strain Data Sheet


Strain Information

BRC No.RBRC01965
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Fbxo15<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Strain developmentDeveloped by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exon 3 through 7. The mutant mice were backcross to C57BL/6J for several times.
Strain descriptionFbxo15, F-box protein 15 is a novel target of undifferentiated-cell-specific transcripton factor Oct3/4 playing essential roles in self-renewal of ES cells and mouse early development. Fbxo15 is predominantly expressed in mouse undiffereniated ES cells. Inactivation of Oct3/4 in ES cells lead to rapid disappearance of Fbxo15 expression. Homozygous mutant mice develop normally and are fertile.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
ReferencesMol. Cell. Biol., 23, 2699-2708 (2003). 12665572

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Fbxo15F-box protein 1518Fbxo15<tm1Yam>targeted mutation 1, Shinya Yamanakaecat3, Fbx15

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)18internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)18

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)18neo; neomycin;

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA18

Ordering Information

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 poly A, mouse Fbxo15 genomic DNA
Research applicationDevelopmental Biology Research
Specific Term and Conditions
DepositorShinya Yamanaka (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: Japanese (Word) / English(Word)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Donato V, Bonora M, Simoneschi D, Sartini D, Kudo Y, Saraf A, Florens L, Washburn MP, Stadtfeld M, Pinton P, Pagano M.
The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem cells.
Nat Cell Biol 19(4) 341-351(2017) 28319092