RBRC01965

Information1

Image
BRC No.RBRC01965
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Fbxo15<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exon 3 through 7. The mutant mice were backcross to C57BL/6J for several times.
Strain descriptionFbxo15, F-box protein 15 is a novel target of undifferentiated-cell-specific transcripton factor Oct3/4 playing essential roles in self-renewal of ES cells and mouse early development. Fbxo15 is predominantly expressed in mouse undiffereniated ES cells. Inactivation of Oct3/4 in ES cells lead to rapid disappearance of Fbxo15 expression. Homozygous mutant mice develop normally and are fertile.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
ReferencesMol. Cell. Biol., 23, 2699-2708 (2003). 12665572

Health Report

Examination Date / Room / Rack2010/12/13Room:3-ARack:K
2010/10/18Room:3-ARack:K
2010/08/23Room:3-ARack:K
2010/06/21Room:3-ARack:K
2010/04/19Room:3-ARack:K
2009/12/14Room:3-ARack:K
2009/10/19Room:3-ARack:K
2009/08/24Room:3-ARack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Fbxo15F-box protein 1518Fbxo15<tm1Yam>targeted mutation 1, Shinya Yamanaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA18

Information2

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 poly A, mouse Fbxo15 genomic DNA
Research applicationDevelopmental Biology Research
Specific Term and Conditions
DepositorShinya Yamanaka (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
MATERIAL TRANSFER AGREEMENT (Japanese (Word) / English(Word)

BRC mice in Publications

Donato V, Bonora M, Simoneschi D, Sartini D, Kudo Y, Saraf A, Florens L, Washburn MP, Stadtfeld M, Pinton P, Pagano M.
The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem?cells.
Nat. Cell Biol. 19(4) 341-351(2017) 28319092