Strain Data Sheet

RBRC01965

Strain Information

Image
BRC No.RBRC01965
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Fbxo15<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exon 3 through 7. The mutant mice were backcross to C57BL/6J for several times.
Strain descriptionFbxo15, F-box protein 15 is a novel target of undifferentiated-cell-specific transcripton factor Oct3/4 playing essential roles in self-renewal of ES cells and mouse early development. Fbxo15 is predominantly expressed in mouse undiffereniated ES cells. Inactivation of Oct3/4 in ES cells lead to rapid disappearance of Fbxo15 expression. Homozygous mutant mice develop normally and are fertile.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
References
Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development.
Yoshimi Tokuzawa, Eiko Kaiho, Masayoshi Maruyama, Kazutoshi Takahashi, Kaoru Mitsui, Mitsuyo Maeda, Hitoshi Niwa, Shinya Yamanaka
Mol. Cell. Biol., 23, 2699-2708 (2003). 12665572

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fbxo15
MGI:1354755		F-box protein 1518Fbxo15<tm1Yam>
MGI:2655776		targeted mutation 1, Shinya Yamanaka
IRES internal ribosomal entry site (EMCV)18
lacZ beta-galactosidase (E. coli)18
neo neomycin resistance gene (E. coli)18
SV40 polyA18

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 poly A, mouse Fbxo15 genomic DNA
    Research applicationDevelopmental Biology Research
    Specific Term and Conditions
    DepositorShinya Yamanaka (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: Japanese (Word) / English(Word)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Donato V, Bonora M, Simoneschi D, Sartini D, Kudo Y, Saraf A, Florens L, Washburn MP, Stadtfeld M, Pinton P, Pagano M.
    The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem cells.
    Nat Cell Biol 19(4) 341-351(2017) 28319092