Strain Data Sheet

RBRC01950

Information1

Image
BRC No.RBRC01950
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Xist<tm1Sado> Tsix<tm1Enl>
Former Common nameX<dc>; Xist/Tsix double KO mouse
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takashi Sado, National Institute of Genetics in 2005.
Strain descriptionXsit/Tsix double knockout mice (X<dc>). An IRES-EGFP cassette was inserted to replace part of exon 1 of Xist gene in mutated J1 ES cells lacking Tsix gene (Tsix<tm1Enl>) in which exon 2 and 3 were replaced with an IRES Beta-geo cassette. Heterozygous mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]
ReferencesDevelopmental Cell, 9, 159-165 (2005). 15992549

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TsixX (inactive)-specific transcript, antisenseXTsix<tm1Enl>targeted mutation 1, En Li

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Xistinactive X specific transcriptsXXist

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)XEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)Xinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)X

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)Xneo; neomycin;

Information2

Donor DNAmouse Xist/Tsix genomic DNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA, Phage P1 loxP, E. coli neo, E. coli lacZ
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell, 9, 159-165 (2005).The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
The RECIPIENT should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
DepositorTakashi Sado (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Feb 2009
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data