BRC No.RBRC01949
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4(Cg)-Tsix<tm1Sado>
Former Common nameTsix SA; Tsix splicing defect mutant mice
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Strain developmentDeveloped by Takashi Sado, National Institute of Genetics in 2006. J1 ES cells were used to generate the mutant mice. Puromycin resistance was excised by crossing with cre transgenic mice.
Strain descriptionTsix gene splicing knockout mice (Tsix<SA>). The splicing acceptor site of Tsix exon 4 was replaced with a fragment containing an IRES-EGFP and a floxed puromycin resistance gene. This mutant line carries modified allele of Tsix locus to avoid the production major splicing variant. Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]
ReferencesDevelopment, 133, 4925-4931 (2006). 17108001

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
TsixX (inactive)-specific transcript, antisenseXTsix<tm1Sado>targeted mutation 1, Takashi Sado

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)XEGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)X

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP


Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA, Phage P1 loxP, mouse Tsix genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 133, 4925-4931 (2006).The RECIPIENT must inform the DEPOSITOR the research project using the
BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to
avoid the conflict of interest with the DEPOSITOR.
The RECIPIENT should contact the DEPOSITOR in the case of application for
any patents with the results from these mice.
DepositorTakashi Sado (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)
Mouse of the Month Feb 2009

BRC mice in Publications

No Data