Strain Data Sheet

RBRC01939

Information1

Image
BRC No.RBRC01939
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Trpv4<tm1Msz>
Former Common nameTRPV4KO
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strainC57BL/6JJcl
Appearanceblack [a/a b/b C/C]
Strain developmentDeveloped by Makoto Suzuki, Jichi Medical School in 2001. The construct was electoroporated into RW4 ES cells derived from 129X1/SvJ. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionTRPV4 (Trpv4, transient receptor potential cation channel, subfamily V, member 4) knockout mice. Exon 4 encodes the ankyrin-repeat domain was replaced with a neomycin resistance gene. The ion channel TRPV4 is a member of the TRP family, acts as a Ca2+-permeable cation channel in response to mechanical stress. Homozygous mutant mice are viable and fertile, but show a reduced responsiveness to pressure sensation and abnormal osmotic regulation.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesJ. Biol. Chem., 278, 22664-22668 (2003). 12692122
Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). 12777254

Health Report

Examination Date / Room / Rack2023/04/10Room:3-ARack:I
2023/01/10Room:3-ARack:I
2022/10/11Room:3-ARack:I
2022/07/11Room:3-ARack:I
2022/04/11Room:3-ARack:I
2022/01/24Room:3-ARack:I
2021/10/26Room:3-ARack:I
2021/08/16Room:3-DRack:D
2021/07/26Room:3-ARack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Trpv4transient receptor potential cation channel, subfamily V, member 45Trpv4<tm1Msz>targeted mutation 1, Makoto Suzuki0610033B08Rik, OTRPC4, Trp12, VR-OAC, VRL-2, VROAC

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Trpv4 genomic DNA, PGK-neo cassette (mouse phosphoglycerate kinase-1 promoter, E. coli aminoglycoside phosphotransferase cDNA)derived from the plasmid pKJ2.
Research applicationSensorineural Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J, Biol. Chem., 278, 22664-22668 (2003). Am. J. Physiol. Cell Physiol., 285, C96-C101(2003).
DepositorMakoto Suzuki (Jichi Medical University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Ueda T, Hoshikawa M, Shibata Y, Kumamoto N, Ugawa S.
Basal cells express functional TRPV4 channels in the mouse nasal epithelium.
Biochem Biophys Rep 4 169-174(2015) 29124201

Weber J, Rajan S, Schremmer C, Chao YK, Krasteva-Christ G, Kannler M, Yildirim AÖ, Brosien M, Schredelseker J, Weissmann N, Grimm C, Gudermann T, Dietrich A.
TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema.
JCI Insight 5(20) (2020) 32931478