Information1 | |
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Image | |
BRC No. | RBRC01939 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.129X1-Trpv4<tm1Msz> |
Former Common name | TRPV4KO |
H-2 Haplotype | |
ES Cell line | RW4 [129X1/SvJ] |
Background strain | C57BL/6JJcl |
Appearance | black [a/a b/b C/C] |
Strain development | Developed by Makoto Suzuki, Jichi Medical School in 2001. The construct was electoroporated into RW4 ES cells derived from 129X1/SvJ. The mutant mice were backcrossed to C57BL/6J. |
Strain description | TRPV4 (Trpv4, transient receptor potential cation channel, subfamily V, member 4) knockout mice. Exon 4 encodes the ankyrin-repeat domain was replaced with a neomycin resistance gene. The ion channel TRPV4 is a member of the TRP family, acts as a Ca2+-permeable cation channel in response to mechanical stress. Homozygous mutant mice are viable and fertile, but show a reduced responsiveness to pressure sensation and abnormal osmotic regulation. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). 12777254 J. Biol. Chem., 278, 22664-22668 (2003). 12692122 |
Health Report | |
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Examination Date / Room / Rack | 2021/02/15Room:3-DRack:D 2020/11/16Room:3-DRack:D 2020/08/24Room:3-DRack:D 2020/06/01Room:3-DRack:D 2020/02/17Room:3-DRack:D 2019/12/16Room:3-DRack:D 2019/10/21Room:3-DRack:D 2019/08/26Room:3-DRack:D |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Trpv4transient receptor potential cation channel, subfamily V, member 45Trpv4<tm1Msz>targeted mutation 1, Makoto Suzuki0610033B08Rik, OTRPC4, Trp12, VR-OAC, VRL-2, VROAC Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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Donor DNA | mouse Trpv4 genomic DNA, PGK-neo cassette (mouse phosphoglycerate kinase-1 promoter, E. coli aminoglycoside phosphotransferase cDNA)derived from the plasmid pKJ2. |
Research application | Sensorineural Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J, Biol. Chem., 278, 22664-22668 (2003). Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). |
Depositor | Makoto Suzuki (Jichi Medical University) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Genotyping protocol -PCR- |
BRC mice in Publications |
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Ueda T, Hoshikawa M, Shibata Y, Kumamoto N, Ugawa S. Basal cells express functional TRPV4 channels in the mouse nasal epithelium. Biochem Biophys Rep 4 169-174(2015) 29124201 |