BRC No.RBRC01939
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Trpv4<tm1Msz>
Former Common nameTRPV4KO
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strainC57BL/6JJcl
Appearanceblack [a/a b/b C/C]
Strain developmentDeveloped by Makoto Suzuki, Jichi Medical School in 2001. The construct was electoroporated into RW4 ES cells derived from 129X1/SvJ. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionTRPV4 (Trpv4, transient receptor potential cation channel, subfamily V, member 4) knockout mice. Exon 4 encodes the ankyrin-repeat domain was replaced with a neomycin resistance gene. The ion channel TRPV4 is a member of the TRP family, acts as a Ca2+-permeable cation channel in response to mechanical stress. Homozygous mutant mice are viable and fertile, but show a reduced responsiveness to pressure sensation and abnormal osmotic regulation.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesJ. Biol. Chem., 278, 22664-22668 (2003). 12692122
Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). 12777254

Health Report

Examination Date / Room / Rack2020/06/01Room:3-DRack:D


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Trpv4transient receptor potential cation channel, subfamily V, member 45Trpv4<tm1Msz>targeted mutation 1, Makoto Suzuki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse Trpv4 genomic DNA, PGK-neo cassette (mouse phosphoglycerate kinase-1 promoter, E. coli aminoglycoside phosphotransferase cDNA)derived from the plasmid pKJ2.
Research applicationSensorineural Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J, Biol. Chem., 278, 22664-22668 (2003).The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR (Dr. Makoto Suzuki). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMakoto Suzuki (Jichi Medical University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Ueda T, Hoshikawa M, Shibata Y, Kumamoto N, Ugawa S.
Basal cells express functional TRPV4 channels in the mouse nasal epithelium.
Biochem Biophys Rep 4 169-174(2015) 29124201