BRC No.RBRC01939
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Trpv4<tm1Msz>
Former Common nameTRPV4KO
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strainC57BL/6JJcl
Appearanceblack [a/a b/b C/C]
Strain developmentDeveloped by Makoto Suzuki, Jichi Medical School in 2001. The construct was electoroporated into RW4 ES cells derived from 129X1/SvJ. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionTRPV4 (Trpv4, transient receptor potential cation channel, subfamily V, member 4) knockout mice. Exon 4 encodes the ankyrin-repeat domain was replaced with a neomycin resistance gene. The ion channel TRPV4 is a member of the TRP family, acts as a Ca2+-permeable cation channel in response to mechanical stress. Homozygous mutant mice are viable and fertile, but show a reduced responsiveness to pressure sensation and abnormal osmotic regulation.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesAm. J. Physiol. Cell Physiol., 285, C96-C101(2003). 12777254
J. Biol. Chem., 278, 22664-22668 (2003). 12692122

Health Report

Examination Date / Room / Rack2021/02/15Room:3-DRack:D


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Trpv4transient receptor potential cation channel, subfamily V, member 45Trpv4<tm1Msz>targeted mutation 1, Makoto Suzuki0610033B08Rik, OTRPC4, Trp12, VR-OAC, VRL-2, VROAC

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse Trpv4 genomic DNA, PGK-neo cassette (mouse phosphoglycerate kinase-1 promoter, E. coli aminoglycoside phosphotransferase cDNA)derived from the plasmid pKJ2.
Research applicationSensorineural Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J, Biol. Chem., 278, 22664-22668 (2003).
Am. J. Physiol. Cell Physiol., 285, C96-C101(2003).
DepositorMakoto Suzuki (Jichi Medical University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Ueda T, Hoshikawa M, Shibata Y, Kumamoto N, Ugawa S.
Basal cells express functional TRPV4 channels in the mouse nasal epithelium.
Biochem Biophys Rep 4 169-174(2015) 29124201