RBRC01939
Information1 | |
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| Image | |
| BRC No. | RBRC01939 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129X1-Trpv4<tm1Msz> |
| Former Common name | TRPV4KO |
| H-2 Haplotype | |
| ES Cell line | RW4 [129X1/SvJ] |
| Background strain | C57BL/6JJcl |
| Appearance | black [a/a b/b C/C] |
| Strain development | Developed by Makoto Suzuki, Jichi Medical School in 2001. The construct was electoroporated into RW4 ES cells derived from 129X1/SvJ. The mutant mice were backcrossed to C57BL/6J. |
| Strain description | TRPV4 (Trpv4, transient receptor potential cation channel, subfamily V, member 4) knockout mice. Exon 4 encodes the ankyrin-repeat domain was replaced with a neomycin resistance gene. The ion channel TRPV4 is a member of the TRP family, acts as a Ca2+-permeable cation channel in response to mechanical stress. Homozygous mutant mice are viable and fertile, but show a reduced responsiveness to pressure sensation and abnormal osmotic regulation. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
| References | J. Biol. Chem., 278, 22664-22668 (2003). 12692122 Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). 12777254 |
Health Report | |
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| Examination Date / Room / Rack | 2020/06/01Room:3-DRack:D 2020/02/17Room:3-DRack:D 2019/12/16Room:3-DRack:D 2019/10/21Room:3-DRack:D 2019/08/26Room:3-DRack:D 2019/06/17Room:3-DRack:D 2019/04/15Room:3-DRack:D 2019/02/18Room:3-DRack:D |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele namePromoter Trpv4transient receptor potential cation channel, subfamily V, member 45Trpv4<tm1Msz>targeted mutation 1, Makoto Suzuki Gene symbolGene nameChr.Allele symbolAllele namePromoter neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | mouse Trpv4 genomic DNA, PGK-neo cassette (mouse phosphoglycerate kinase-1 promoter, E. coli aminoglycoside phosphotransferase cDNA)derived from the plasmid pKJ2. |
| Research application | Sensorineural Research |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J, Biol. Chem., 278, 22664-22668 (2003).The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR (Dr. Makoto Suzuki). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Makoto Suzuki (Jichi Medical University) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Genotyping protocol -PCR- |
BRC mice in Publications |
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Ueda T, Hoshikawa M, Shibata Y, Kumamoto N, Ugawa S. Basal cells express functional TRPV4 channels in the mouse nasal epithelium. Biochem Biophys Rep 4 169-174(2015) 29124201 |