RBRC01926

Information1

Image
BRC No.RBRC01926
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Vamp7<tm1Aha>
Former Common nameVamp7 KO, B6;129-Vamp7<tm1>
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Akihiro Harada, Institute for Molecular and Cellular Regulation, Gunma University in 2005. Two lines were generated (RBRC01926 and RBRC04855).
Strain descriptionVamp7 floxed mice (Neo type). Exons 3 and 4 of the Vamp7 gene were flanked by loxP sites, an FRT-SA-Ex 5,6,7,8-IRES-Neo-polyA-FRT cassette was inserted between exons 4 and 5. Vamp7 is a member of VAMP (vesicle-associated membrane protein) subfamily of SNARES proteins which primarily mediate fusion of cellular transport vesicles with a target membrane, and ubiquitously expressed and in localized to the late endsome, the lysosome, and the trans-Golgi network (TGN).
Colony maintenanceHomozygote x HemizygoteHomozgous females and hemizygous males are viable and fertile.
ReferencesJ. Cell Biol., 2000 May 15;149(4):889-900. 10811829
Mol. Biol. Cell, 9, 1437-1448 (1998). 9614185
Traffic., 12(10):1383-1393 (2011). 21740490

Health Report

Examination Date / Room / Rack2013/11/11Room:2-検Rack:N
2013/09/09Room:2-検Rack:N
2013/07/08Room:2-検Rack:N
2013/05/13Room:2-検Rack:N
2013/03/04Room:2-検Rack:N
2013/01/07Room:2-検Rack:N
2011/08/01Room:3-CRack:A
2011/06/20Room:3-CRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Vamp7vesicle-associated membrane protein 7XVamp7<tm1Aha>targeted mutation 1, Akihiro Harada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)X

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)X

Information2

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli neo, phage P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse VAMP7 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Within a period of TWO years after the first paper concerning the analysis of the BIOLOGICAL RESOURCE is published, the RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorAkihiro Harada (Gunma University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data