RBRC01923

Information1

Image
BRC No.RBRC01923
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Zeb1<tm2Yhi>/YhiRbrc
Former Common namedelta EF1 Knockout mice, delta EF1 null (lacZ), B6;129-Zfhx1a<tm2Yhi>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yujiro Higashi, Institute for Molecular and Cellular Biology, Osaka University. R1 ES cells were used. The mutant mice were crossed to C57BL/6.
Strain descriptionZeb1 gene knockout mice (deltaEF1 null lacZ). Exon 1 was replaced with a lacZ-neo cassette including termination signals. Homozygous mutant mice die shortly after birth.
Colony maintenance
ReferencesDevelopment, 125, 21-31 (1998). 9389660

Health Report

Examination Date / Room / Rack2011/12/12Room:3-CRack:L
2011/10/17Room:3-CRack:L
2011/08/29Room:3-CRack:L
2011/06/20Room:3-CRack:L
2011/04/18Room:3-CRack:L
2011/02/21Room:3-CRack:L
2010/12/13Room:3-CRack:L
2010/10/18Room:3-CRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Zeb1zinc finger E-box binding homeobox 118Zeb1<tm2Yhi>targeted mutation 2, Yujiro Higashi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)18SV40 early gene promoter and termination sequence (SV40), Thymidine kinase gene promoter sequence (HSV)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neomycin resistance gene coding sequence, E. coli LacZ gene coding sequence, SV40 early gene promoter and termination sequence, mouse Zeb1 genomic DNA
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 125, 21-31 (1998).The RECIPIENT should consult with the DEPOSITOR in the case of application for any patents or commercial use with the results derived from the use of the BIOLOGICAL RESOURCE. The RECIPIENT should consult with the DEPOSITOR on the authorship upon publications of the results obtained from the use of the BIOLOGICAL RESOURCE. The authorship should be considered in accordance to generally accepted practices and decided through agreement between the RECIPIENT and the DEPOSITOR/DEVELOPER.
DepositorYujiro Higashi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data