RBRC01922

Information1

Image
BRC No.RBRC01922
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Zeb1<tm1Yhi>
Former Common nameδ EF1 [Delta]C-fin, δ EF1 [Delta]C727
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yujiro Higashi, Institute for Molecular and Cellular Biology, Osaka University. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 11 times.
Strain descriptionZeb1 gene knockout mice (deltaEF1 deltaC727). Exon 6 of the Zeb1 was replaced with a neo cassette. In the genetic background of inbred mouse strains, homozygous mutant mice die after birth. While about 20-30% of homozygous mice survive to adulthood in the mixed genetic background.
Colony maintenance
ReferencesJ. Exp. Med., 185, 1467-1480 (1997). 9126927

Health Report

Examination Date / Room / Rack2009/02/23Room:3-CRack:I
2008/12/15Room:3-CRack:I
2008/10/20Room:3-CRack:I
2008/08/25Room:3-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Zeb1zinc finger E-box binding homeobox 118Zeb1<tm1Yhi>targeted mutation 1, Yujiro Higashi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18SV40 early promoter and herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAneomycin resistance gene coding sequence (E. coli), SV40 early gene promoter and termination sequence (SV40), Thymidine kinase gene promoter sequence (HSV)
Research applicationDevelopmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 185, 1467-1480 (1997).The RECIPIENT should consult with the DEPOSITOR in the case of application for any patents or commercial use with the results derived from the use of the BIOLOGICAL RESOURCE.
DepositorYujiro Higashi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data