Strain Data Sheet

RBRC01916

Information1

Image
BRC No.RBRC01916
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Rnf103<tm1.1Thg>/ThgRbrc
Former Common nameHicky mouse, pKF1KO4.3.1
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Dr. Tamotsu Hashimoto-Gotoh at Kyoto Prefectural University of Medicine in 2001.
Strain descriptionThe kf-1 gene was first discovered as a cDNA clone whose mRNA synthesis is elevated in the cerebral cortex of Alzheimer's disease patient (1), and identified later in the rat cerebral cortex after repeated electroconvulsive treatment and chronic antidepressant treatment with SSRI (2). The KF-1 protein is one of the Zinc finger proteins belonging to the RING H2 family, which have been assessed for their ability to facilitate E2-dependent ubiquitination (3). Cells that express exogenous kf-1 result in apoptotic cell death in vitro (unpublished data). Kf-1 deficient mice, called "hicky" mice ("hicky" means hikikomori symptome in Japanese slung, which resembles to "social withdrawal" in English.), display typical anxiety-like behaviors without any other major abnormalities including pathological, physical, behavioral and reproductive aspects.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NJcl)
ReferencesFront. Behav. Neurosci., 2, 3389 (2008). 18958194

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rnf103ring finger protein 1036Rnf103<tm1.1Thg>targeted mutation 1.1, Tamotsu Hashimoto-Gotohkf-1, Zfp103

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Information2

Donor DNAphage P1 loxP, mouse Rnf103 genomic DNA
Research applicationCre/loxP system
Mouse Models for Human Disease
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front. Behav. Neurosci., 2, 3389 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorTamotsu Hashimoto-Gotoh (Kyoto Prefectural University of Medicine)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Apr 2007
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Tsujimura A, Matsuki M, Takao K, Yamanishi K, Miyakawa T, Hashimoto-Gotoh T.
Mice lacking the kf-1 gene exhibit increased anxiety- but not despair-like behavior.
Front Behav Neurosci 2 4(2008) 18958194