Strain Data Sheet

RBRC01897

Strain Information

Image
BRC No.RBRC01897
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Sox2<tm1Okud>
Former Common nameFloxed Sox2 mice
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Akihiko Okuda, Research Center for Genomic Medicine, Saitama Medical University in 2004. C57BL/6 background.
Strain descriptionSox2 gene is a member of the SRY-related HMG box gene family that encode transcription factors with a single HMG DNA-binding domain, expresses in multipotent neural stem cells at all stages of mouse ontogeny. Sox2 ORF was replaced with IREShygTKpA cassette flanked by loxP sites. Conditional Sox2 deficient mice can be generated by crossing this mice with tissue-specific Cre mice.
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]. Fertility of heterozygous male is low.
References
Consequence of the loss of Sox2 in the developing brain of the mouse.
Miyagi S, Masui S, Niwa H, Saito T, Shimazaki T, Okano H, Nishimoto M, Muramatsu M, Iwama A, Okuda A
FEBS Lett., 582,　2811-2815 (2008). 18638478

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRESinternal ribosomal entry site (EMCV)3
  • anophthalmia/microphthalmia-esophageal atresia syndrome(MedGEN)
    • Sox2SRY (sex determining region Y)-box 23Sox2targeted mutation 1, Akihiko Okuda
  • anophthalmia/microphthalmia-esophageal atresia syndrome(MedGEN)
    • loxPphage P1 loxP3loxP
  • anophthalmia/microphthalmia-esophageal atresia syndrome(MedGEN)
    • loxPphage P1 loxP3loxP
  • anophthalmia/microphthalmia-esophageal atresia syndrome(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal neuronal precursor proliferation(MP:0004948)

  • enlarged lateral ventricles(MP:0008535)

  • neonatal lethality, complete penetrance(MP:0011087)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, Encephalomyocorditis virus (EMCV) internal ribosomal entrysite (IRES), E. coli hygromycin phosphotransferase, herpes simplex virus thymidine kinase polyA, mouse Sox2 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
    DepositorAkihiko Okuda (Saitama Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Sep 2009

    BRC mice in Publications

    No Data