RBRC01897

Information1

Image
BRC No.RBRC01897
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Sox2<tm1Okud>
Former Common nameFloxed Sox2 mice
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Akihiko Okuda, Research Center for Genomic Medicine, Saitama Medical University in 2004. C57BL/6 background.
Strain descriptionSox2 gene is a member of the SRY-related HMG box gene family that encode transcription factors with a single HMG DNA-binding domain, expresses in multipotent neural stem cells at all stages of mouse ontogeny. Sox2 ORF was replaced with IREShygTKpA cassette flanked by loxP sites. Conditional Sox2 deficient mice can be generated by crossing this mice with tissue-specific Cre mice.
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]. Fertility of heterozygous male is low.
ReferencesFEBS Lett., 582, 2811-2815 (2008). 18638478

Health Report

Examination Date / Room / Rack2014/07/14Room:4-ARack:F
2014/05/19Room:4-ARack:F
2014/03/24Room:4-ARack:F
2014/01/20Room:4-ARack:F
2014/01/08Room:4-ARack:F
2013/11/25Room:4-ARack:F
2013/11/25Room:4-ARack:F
2013/09/24Room:4-ARack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sox2SRY-box containing gene 23Sox2<tm1Okud>targeted mutation 1, Akihiko Okuda

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)3

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP3loxP

Information2

Donor DNAPhage P1 loxP sites, Encephalomyocorditis virus (EMCV) internal ribosomal entrysite (IRES), E. coli hygromycin phosphotransferase, herpes simplex virus thymidine kinase polyA, mouse Sox2 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
DepositorAkihiko Okuda (Saitama Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Sep 2009

BRC mice in Publications

No Data