RBRC01894

Information1

Image
BRC No.RBRC01894
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Mgl2<tm1Tats>/TatsRbrc
Former Common namemMgl2 knockout mice (BALB/c)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cAnNCrlCrlj
Appearance
Strain developmenteveloped by Kaori Denda and Tasturo Irimura, Graduate School of Pharmaceutical Sciences, The University of Tokyo. TT2 ES cells derived from B6CBAF1 were used to generate the knockout mice. The mice were backcrossed to C57BL/6J and BALB/c, respectively (RBRC01893 and RBRC01894).
Strain descriptionMgl2 gene knockout mice. Exon 2 of the Mgl2 was replaced with a PGK-neo cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. Homozygous mutant mice are viable and fertile. Under investigation (details unknown). C57BL/6 background (RBRC01893) and BALB/c background (RBRC01894).
Colony maintenanceHomozygote x Homozygote [or Crossing to BALB/cAnNCrlCrlj]
References

Health Report

Examination Date / Room / Rack2013/07/22Room:4-BRack:F
2013/05/20Room:4-BRack:F
2013/03/25Room:4-BRack:F
2013/01/21Room:4-BRack:F
2012/11/26Room:4-BRack:F
2012/09/24Room:4-BRack:F
2012/07/23Room:4-BRack:F
2012/05/21Room:4-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mgl2macrophage galactose N-acetyl-galactosamine specific lectin 211Mgl2<tm1Tats>targeted mutation 1, Tatsuro Irimura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine growth hormone poly A, mouse Mgl2 genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorTatsuro Irimura (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Lab HP
Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

No Data