Strain Data Sheet

RBRC01893

Strain Information

Image
BRC No.RBRC01893
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mgl2<tm1Tats>/TatsRbrc
Former Common namemMgl2 knockout mice (C57BL/6J)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6J
Appearance
Strain developmenteveloped by Kaori Denda and Tasturo Irimura, Graduate School of Pharmaceutical Sciences, The University of Tokyo. TT2 ES cells derived from B6CBAF1 were used to generate the knockout mice. The mice were backcrossed to C57BL/6J and BALB/c, respectively (RBRC01893 and RBRC01894).
Strain descriptionMgl2 gene knockout mice. Exon 2 of the Mgl2 was replaced with a PGK-neo cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. Homozygous mutant mice are viable and fertile. Under investigation (details unknown). C57BL/6 background (RBRC01893) and BALB/c background (RBRC01894).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GHGrowth hormone polyA (Bovine)11GH
Mgl2macrophage galactose N-acetyl-galactosamine specific lectin 211Mgl2targeted mutation 1, Tatsuro Irimura
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal T-helper 2 cell morphology(MP:0002434)

  • abnormal immune system physiology(MP:0001790)

  • abnormal lymph node size(MP:0000701)

  • decreased B cell number(MP:0005017)

  • decreased CD4-positive, alpha-beta T cell number(MP:0008075)
    • more 7 phenotypes
  • decreased CD8-positive, alpha-beta T cell number(MP:0008079)

  • decreased Langerhans cell number(MP:0008119)

  • decreased dendritic cell number(MP:0008127)

  • decreased interleukin-4 secretion(MP:0008700)

  • increased susceptibility to parasitic infection(MP:0005027)

  • mortality/aging(MP:0010768)

  • reproductive system phenotype(MP:0005389)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine growth hormone poly A, mouse Mgl2 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorTatsuro Irimura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data