RBRC01892

Information1

Image
BRC No.RBRC01892
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.B6(129)-Clec10a<tm1Hed>/TatsRbrc
Former Common namemMgl1 knockout mice (BALB/c)
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainBALB/cAnNCrlCrlj
Appearance
Strain developmentDeveloped by Stephen M Hedrick, University of California. R1 ES cells were used to generate the knockout mice. R RBRC01891: backcrossed to C57BL/6J over 8 times. RBRC01892: backcrossed to C57BL/6J for 8 times, and then backcrossed to BALB/c over 6 times.
Strain descriptionMgl1 gene knockout mice. Exons 2 and 3 were replaced with a PGK-neo cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. C57BL/6 background (RBRC01891) and BALB/c mixed background (RBRC01892). Homozygous mutant mice were viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to BALB/cAnNCrlCrlj]
ReferencesMol. Cell. Biol., 22, 5173-5181 (2002). 12077344

Health Report

Examination Date / Room / Rack2015/01/21Room:4-BRack:D
2015/01/19Room:4-BRack:D
2015/01/19Room:4-BRack:D
2014/11/17Room:4-BRack:D
2014/09/16Room:4-BRack:D
2014/07/30Room:4-BRack:D
2014/07/28Room:4-BRack:D
2014/07/14Room:4-BRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Clec10amacrophage galactose N-acetyl-galactosamine specific lectin 111Clec10a<tm1Hed>targeted mutation 1, Stephen M Hedrick

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine growth hormone poly A, mouse Clec10a genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorTatsuro Irimura (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data