Strain Data Sheet


Strain Information

BRC No.RBRC01888
TypeSpontaneous Mutation Congenic
SpeciesMus musculus
Strain nameC57BL/6-W<sh>/W<sh>
Former Common nameW sash, C57BL/6-W<sh>
H-2 Haplotype
ES Cell line
Background strainC57BL/6J
Appearancewhite, black eyes [a/a B/B C/C Sh/Sh]
black [a/a B/B C/C +/+]
black with white spotting [a/a B/B C/C Sh/+]
Strain developmentDeveloped by Keisuke Oboki, RIKEN Research Center for Allergy anf Immunology. This strain was generated by backcrossing to C57BL/6 from C;B6-a/a-b/b-W<sh>/W<sh> strain (RBRC00762).
Strain descriptionThe Kit<W-sh> mutation arose spontaneously in the (C3H/HeN x 101/H)F1 at the MRC (Lyon et al., 1982). Homozygous mice are white coat with black eyes. Heterozygotes are black with a white sash. This strain is useful for study of melanogenesis and neural crest migration.
Colony maintenanceHeteryozygote x Homozygote. Homozygous mutant mice are viable and fertile.
ReferencesBlood, 80, 1448-1453 (1992). 1381627
Am. J. Pathal., 167, 835-848 (2005). 16127161
Blood, 83, 3509-3516 (1994). 7515715
Development, 118, 705-717 (1993). 7521281
Blood, 94, 2658-2666 (1999). 10515869
Genet. Res., 39, 315-322 (1982). 7117838

Health Report

Examination Date / Room / Rack2024/06/10Room:3-6Rack:A


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kitkit oncogene5Kit<W-Sh>sashbelly-spot, c-KIT, CD117, Dominant white spotting, Steel Factor Receptor, Tr-kit

Ordering Information

Donor DNA
Research applicationDermatology Research
Immunology and Inflammation Research
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorKeisuke Oboki (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Iwanaga K, Nakamura T, Maeda S, Aritake K, Hori M, Urade Y, Ozaki H, Murata T.
Mast cell-derived prostaglandin D2 inhibits colitis and colitis-associated colon cancer in mice.
Cancer Res 74(11) 3011-9(2014) 24879565

Schuler P, Contassot E, Irla M, Hugues S, Preynat-Seauve O, Beermann F, Donda A, French LE, Huard B.
Direct presentation of a melanocyte-associated antigen in peripheral lymph nodes induces cytotoxic CD8+ T cells.
Cancer Res 68(20) 8410-8(2008) 18922914

Murata A, Hikosaka M, Yoshino M, Zhou L, Hayashi SI.
Kit-independent mast cell adhesion mediated by Notch.
Int Immunol 31(2) 69-79(2019) 30299470

Hitomi K, Tahara-Hanaoka S, Miki H, Iwata K, Shibayama S, Kubo M, Shibuya A.
Allergin-1 on mast cells suppresses house dust mite-induced airway hyperresponsiveness in mice.
Int Immunol 30(9) 429-434(2018) 30169732

Nakahashi-Oda C, Tahara-Hanaoka S, Shoji M, Okoshi Y, Nakano-Yokomizo T, Ohkohchi N, Yasui T, Kikutani H, Honda S, Shibuya K, Nagata S, Shibuya A.
Apoptotic cells suppress mast cell inflammatory responses via the CD300a immunoreceptor.
J Exp Med 209(8) 1493-503(2012) 22826299

Ohneda K, Moriguchi T, Ohmori S, Ishijima Y, Satoh H, Philipsen S, Yamamoto M.
Transcription factor GATA1 is dispensable for mast cell differentiation in adult mice.
Mol Cell Biol 34(10) 1812-26(2014) 24615013

Nakamura T, Maeda S, Horiguchi K, Maehara T, Aritake K, Choi BI, Iwakura Y, Urade Y, Murata T.
PGD2 deficiency exacerbates food antigen-induced mast cell hyperplasia.
Nat Commun 6 7514(2015) 26159556

Murata T, Aritake K, Matsumoto S, Kamauchi S, Nakagawa T, Hori M, Momotani E, Urade Y, Ozaki H.
Prostagladin D2 is a mast cell-derived antiangiogenic factor in lung carcinoma.
Proc Natl Acad Sci U S A 108(49) 19802-7(2011) 22106279

Yoshida K, Tanihara S, Miyashita Y, Obayashi K, Ito MA, Yamamoto K, Imai T, Matsuoka I.
P2X4 receptor stimulation enhances MrgprB2-mediated mast cell activation and pseudoallergic reactions in mice.
Sci Rep 12(1) 18613(2022) 36329102