Strain Data Sheet

RBRC01862

Strain Information

Image
BRC No.RBRC01862
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp2<tm(GFP)>/YsaRbrc
Former Common nameMesp2-GFP
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Cooperative Mesp activity is required for normal somitogenesis along the anterior-posterior axis.
Morimoto M, Kiso M, Sasaki N, Saga Y
Dev. Biol., 300, 687-698 (2006). 16996494

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)7
  • spondylocostal dysostosis 1, autosomal recessive(MedGEN)

  • spondylocostal dysostosis 2, autosomal recessive(MedGEN)
    • GHGrowth hormone polyA (Bovine)7GH
  • spondylocostal dysostosis 1, autosomal recessive(MedGEN)

  • spondylocostal dysostosis 2, autosomal recessive(MedGEN)
    • Mesp2mesoderm posterior 27Mesp2targeted mutation 11, Yumiko Saga
  • spondylocostal dysostosis 1, autosomal recessive(MedGEN)

  • spondylocostal dysostosis 2, autosomal recessive(MedGEN)
    • loxPphage P1 loxP7loxP
  • spondylocostal dysostosis 1, autosomal recessive(MedGEN)

  • spondylocostal dysostosis 2, autosomal recessive(MedGEN)
    • loxPphage P1 loxP7loxP
  • spondylocostal dysostosis 1, autosomal recessive(MedGEN)

  • spondylocostal dysostosis 2, autosomal recessive(MedGEN)
    • neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)
  • spondylocostal dysostosis 1, autosomal recessive(MedGEN)

  • spondylocostal dysostosis 2, autosomal recessive(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal intervertebral disk morphology(MP:0004173)

  • abnormal skeleton development(MP:0002113)

  • abnormal vertebral pedicle morphology(MP:0004604)

  • decreased body height(MP:0001255)

  • postnatal lethality, complete penetrance(MP:0011085)
    • more 2 phenotypes
  • rib fusion(MP:0000154)

  • vertebral fusion(MP:0004609)
    • Detailed phenotype data

    Ordering Information

    Donor DNAJellyfish GFP cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine GH polyA, mouse mesp2 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 300, 687-698 (2006).
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Lauschke VM, Tsiairis CD, François P, Aulehla A.
    Scaling of embryonic patterning based on phase-gradient encoding.
    Nature 493(7430) 101-5(2013) 23254931