RBRC01858

Information1

Image
BRC No.RBRC01858
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Tubg2<tm1Sts>/StsRbrc
Former Common nameTUBG2 knockout mouse
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Drs. Akiko Yuba-Kubo and Shioichiro Tsukita at Kyoto University in 2001. A Pgk-neo cassette was inserted to replace exon 5 og Tobg2 gene. The mutant mice were backcrossed to C57BL/6 over 6 generation.
Strain descriptionγ-tubulin play a central role in the nucleaton of microtubules. Tubg2 is a γ-tubulin isoforms and primarily detected in the brain. Tubg2 homozyous deficient mice are viable, but sometimes become sterile with age by impared motor function.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesDev. Biol., 282, 361-373 (2005). 15893303

Health Report

Examination Date / Room / Rack2010/07/12Room:4-BRack:K
2010/05/17Room:4-BRack:K
2010/03/15Room:4-BRack:K
2010/01/18Room:4-BRack:K
2007/07/17Room:4-ARack:G
2007/05/22Room:4-ARack:G
2007/05/21Room:4-ARack:G
2007/03/19Room:4-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tubg2tubulin, gamma 211Tubg2<tm1Sts>targeted mutation 1, Shoichiro Tsukita

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Tubg2 genomic DNA
Research applicationDevelopmental Biology Research
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkiko Kubo (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data