Strain Data Sheet



BRC No.RBRC01834
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-flpe)36Ito/ItoRbrc
Former Common nameB6-Tg(CAG-FLPe)36; C57BL/6-Tg(CAG-FLPe)36Ito/ItoRbrc
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeposited by Hiroaki Kanki and Shigeyoshi Itohara, RIKEN Brain Science Institute in 2006. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6J background.
Strain descriptionThis transgenic mouse of C57BL/6 strain expresses the FLPe recombinase under the control of the CAG promoter. FLPe is a variant of the FLP recombinase with a higher enzymatic activity at 37 degrees C. The FLP-FRT system is similar to the cre-loxP system and is useful for more complex dual approaches that combine FLP with other recombinases, such as Cre and phi C31. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have a pair of FLP recombinase target (FRT) sequence flanking the genomic region of interest.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesExp. Anim., 55, 137-141 (2006). 16651697

Health Report

Examination Date / Room / Rack2021/09/08Room:4-ARack:F


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLPeyeast flippase recombinaseUNFLPeCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UNinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin resistance gene (Streptomyces alboniger)UNpuro


Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), yeast FLPe cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), Streptomyces alboniger puromycin resistance gene
Research applicationFLP/frt system
General Purpose
Genetics Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Experimental Animals, 55, 137-141 (2006).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Mouse of the Month Feb 2007
Genetic Background
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Yanagihara T, Tomino T, Uruno T, Fukui Y.
Thymic epithelial cell-specific deletion of Jmjd6 reduces Aire protein expression and exacerbates disease development in a mouse model of autoimmune diabetes.
Biochem. Biophys. Res. Commun. 489(1) 8-13(2017) 28546003

Sugimoto M, Shimizu Y, Zhao S, Ukon N, Nishijima K, Wakabayashi M, Yoshioka T, Higashino K, Numata Y, Okuda T, Tamaki N, Hanamatsu H, Igarashi Y, Kuge Y.
Characterization of the role of sphingomyelin synthase 2 in glucose metabolism in whole-body and peripheral tissues in mice.
Biochim. Biophys. Acta (2016) 27151272

Nakata Y, Ueda T, Nagamachi A, Yamasaki N, Ikeda KI, Sera Y, Takubo K, Kanai A, Oda H, Sanada M, Ogawa S, Tsuji K, Ebihara Y, Wolff L, Honda ZI, Suda T, Inaba T, Honda H.
Acquired expression of Cbl(Q367P) in mice induces dysplastic myelopoiesis mimicking chronic myelomonocytic leukemia.
Blood 129(15) 2148-2160(2017) 28209720

Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald JA, Kimata K, Taniguchi S, Itano N.
Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization.
Cancer Res. 70(18) 7073-83(2010) 20823158

Nakahara R, Kawai Y, Oda A, Nishimura M, Murakami A, Azuma T, Kaifu T, Goitsuka R.
Generation of a Tlx1CreER-Venus knock-in mouse strain for the study of spleen development.
Genesis (2014) 25283275

Herndler-Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, Lietzenmayer M, Kroehling L, Takumi A, Kometani K, Inoue T, Kluger Y, Kaech SM, Kurosaki T, Okada T, Flavell RA.
KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity.
Immunity (2018) 29625895

Okuma A, Hoshino K, Ohba T, Fukushi S, Aiba S, Akira S, Ono M, Kaisho T, Muta T.
Enhanced apoptosis by disruption of the STAT3-IkappaB- signaling pathway in epithelial cells induces Sj gren's syndrome-like autoimmune disease.
Immunity 38(3) 450-60(2013) 23453632

Tsunoda T, Doi K, Ishikura S, Luo H, Nishi K, Matsuzaki H, Koyanagi M, Tanaka Y, Okamura T, Shirasawa S.
Zfat expression in ZsGreen reporter gene knock?in mice: Implications for a novel function of Zfat in definitive erythropoiesis.
Int. J. Mol. Med. (2018) 30106088

Hara T, Mimura K, Abe T, Shioi G, Seiki M, Sakamoto T.
Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock.
J. Biol. Chem. 286(37) 32542-51(2011) 21778228

Fumoto T, Takeshita S, Ito M, Ikeda K.
Physiological functions of osteoblast lineage and T cell-derived RANKL in bone homeostasis.
J. Bone Miner. Res. (2013) 24014480

Kaji T, Ishige A, Hikida M, Taka J, Hijikata A, Kubo M, Nagashima T, Takahashi Y, Kurosaki T, Okada M, Ohara O, Rajewsky K, Takemori T.
Distinct cellular pathways select germline-encoded and somatically mutated antibodies into immunological memory.
J. Exp. Med. (2012) 23027924

Kimura-Ueki M, Oda Y, Oki J, Komi-Kuramochi A, Honda E, Asada M, Suzuki M, Imamura T.
Hair cycle resting phase is regulated by cyclic epithelial FGF18 signaling.
J. Invest. Dermatol. 132(5) 1338-45(2012) 22297635

Ogino H, Hisanaga A, Kohno T, Kondo Y, Okumura K, Kamei T, Sato T, Asahara H, Tsuiji H, Fukata M, Hattori M.
Secreted Metalloproteinase ADAMTS-3 Inactivates Reelin.
J. Neurosci. 37(12) 3181-3191(2017) 28213441

Kai T, Tsukamoto Y, Hijiya N, Tokunaga A, Nakada C, Uchida T, Daa T, Iha H, Takahashi M, Nomura T, Sato F, Mimata H, Ikawa M, Seto M, Matsuura K, Moriyama M.
Kidney-specific knockout of Sav1 in the mouse promotes hyper-proliferation of renal tubular epithelium through suppression of the Hippo pathway.
J. Pathol. (2016) 26913567

Kiyozumi D, Taniguchi Y, Nakano I, Toga J, Yagi E, Hasuwa H, Ikawa M, Sekiguchi K.
Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality.
Life Sci Alliance (2018) 30456378

Kawakami E, Tokunaga A, Ozawa M, Sakamoto R, Yoshida N.
The histone demethylase fbxl11/kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators.
Mech. Dev. (2014) 25463925

Kimura-Yoshida C, Mochida K, Nakaya MA, Mizutani T, Matsuo I.
Cytoplasmic localization of GRHL3 upon epidermal differentiation triggers cell shape change for epithelial morphogenesis.
Nat Commun 9(1) 4059(2018) 30283008

Raveney BJ, Oki S, Hohjoh H, Nakamura M, Sato W, Murata M, Yamamura T.
Eomesodermin-expressing T-helper cells are essential for chronic neuroinflammation.
Nat Commun (2015) 26436530

Maeda K, Kobayashi Y, Udagawa N, Uehara S, Ishihara A, Mizoguchi T, Kikuchi Y, Takada I, Kato S, Kani S, Nishita M, Marumo K, Martin TJ, Minami Y, Takahashi N.
Wnt5a-Ror2 signaling between osteoblast-lineage cells and osteoclast precursors enhances osteoclastogenesis.
Nat. Med. 18(3) 405-12(2012) 22344299

Kawai Y, Oda A, Kanai Y, Goitsuka R.
Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.
PLoS ONE (2018) 29293683

Nagamachi A, Nakata Y, Ueda T, Yamasaki N, Ebihara Y, Tsuji K, Honda Z, Takubo K, Suda T, Oda H, Inaba T, Honda H.
Acquired deficiency of A20 results in rapid apoptosis, systemic inflammation, and abnormal hematopoietic stem cell function.
PLoS ONE (2014) 24498102

Ikeda K, Ueda T, Yamasaki N, Nakata Y, Sera Y, Nagamachi A, Miyama T, Kobayashi H, Takubo K, Kanai A, Oda H, Wolff L, Honda Z, Ichinohe T, Matsubara A, Suda T, Inaba T, Honda H.
Maintenance of the functional integrity of mouse hematopoiesis by EED and promotion of leukemogenesis by EED haploinsufficiency.
Sci Rep Jul 19;6:29454. doi: 10.1038/srep29454. (2016) 27432459