Strain Data Sheet

RBRC01832

Strain Information

Image
BRC No.RBRC01832
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Blimp1-RFP)3Rbrc
Former Common nameBlimp1-RFP#3
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Drs. Michihiko Sugimoto and Kuniya Abe, RIKEN BioResource Center in 2005.
Strain descriptionThis transgenic mouse expresses RFP under the control of Blimp1 promoter. Expression intensity display high in line 1 (RBRC01830), weak in line 2 (RBRC01831), line 3 (RBRC01832) and line 4 (RBRC01833) (expression of line 4 is higher than line 2 and 3). Tg Homozygous mice of line 1 are embryonic lethal.
Colony maintenance
References
X chromosome reactivation initiates in nascent primordial germ cells in mice.
Sugimoto M, Abe K
PLoS Genet., 3, e116 (2007). 17676999

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) siteUNFrt
GHGrowth hormone polyA (Bovine)UNGH
mRFPmonomeric Red Fluorescent Protein (Discosoma sp.)UNmRFPmouse Blimp1 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Blimp1 genomic DNA (BAC clone RP23-1D12), mRFP cDNA (Discosoma sp.), bovine growth hormone poly A, yeast FRT (flipase recombination target) site
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 3, e116 (2007).
    DepositorKuniya Abe (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. UCSD MTA
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data