Strain Data Sheet

RBRC01830

Information1

Image
BRC No.RBRC01830
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Blimp1-RFP)1Rbrc
Former Common nameBlimp1-RFP#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Drs. Michihiko Sugimoto and Kuniya Abe, RIKEN BioResource Center in 2005.
Strain descriptionThis transgenic mouse expresses RFP under the control of Blimp1 promoter. Expression intensity display high in line 1 (RBRC01830), weak in line 2 (RBRC01831), line 3 (RBRC01832) and line 4 (RBRC01833) (expression of line 4 is higher than line 2 and 3). Tg Homozygous mice of line 1 are embryonic lethal.
Colony maintenance
ReferencesPLoS Genet., 3, e116 (2007). 17676999

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mRFPmonomeric Red Fluorescent Protein (Discosoma sp.)UNmRFPmouse Blimp1 promoter region, bovine growth hormone poly A

Information2

Donor DNAmouse Blimp1 genomic DNA (BAC clone RP23-1D12), mRFP cDNA (Discosoma sp.), bovine growth hormone poly A, yeast FRT (flipase recombination target) site
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 3, e116 (2007).
DepositorKuniya Abe (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. UCSD MTA
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

Mochizuki K, Hayashi Y, Sekinaka T, Otsuka K, Ito-Matsuoka Y, Kobayashi H, Oki S, Takehara A, Kono T, Osumi N, Matsui Y.
Repression of Somatic Genes by Selective Recruitment of HDAC3 by BLIMP1 Is Essential for Mouse Primordial Germ Cell Fate Determination.
Cell Rep 24(10) 2682-2693.e6(2018) 30184502

Cao L, Shitara H, Sugimoto M, Hayashi J, Abe K, Yonekawa H.
New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice.
PLoS Genet 5(12) e1000756(2009) 19997484