BRC No.RBRC01828
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-Cre)CZ-MO2Osb
Former Common nameCAG-cre
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2003. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were backcrossed C57BL/6N.
Strain descriptionThese transgenic mice express Cre recombinase gene under the control of CAG promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have a loxP site-flanked sequence of interest. The recombination occurs in all over the body.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrSlc]
ReferencesBiochem. Biophys. Res. Commun., 321, 275-279 (2004). 15358172

Health Report

Examination Date / Room / Rack2021/02/15Room:3-CRack:H


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcreCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)


Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 Cre recombinase
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 321, 275-279 (2004).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genetic Background
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Miyasaka Y, Uno Y, Yoshimi K, Kunihiro Y, Yoshimura T, Tanaka T, Ishikubo H, Hiraoka Y, Takemoto N, Tanaka T, Ooguchi Y, Skehel P, Aida T, Takeda J, Mashimo T.
CLICK: one-step generation of conditional knockout mice.
BMC Genomics (2018) 29720086

Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald JA, Kimata K, Taniguchi S, Itano N.
Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization.
Cancer Res. 70(18) 7073-83(2010) 20823158

Maruyama T, Baba T, Maemoto Y, Hara-Miyauchi C, Hasegawa-Ogawa M, Okano HJ, Enda Y, Matsumoto K, Arimitsu N, Nakao K, Hamamoto H, Sekimizu K, Ohto-Nakanishi T, Nakanishi H, Tokuyama T, Yanagi S, Tagaya M, Tani K.
Loss of DDHD2, whose mutation causes spastic paraplegia, promotes reactive oxygen species generation and apoptosis.
Cell Death Dis 9(8) 797(2018) 30038238

Liu T, Yamaguchi Y, Shirasaki Y, Shikada K, Yamagishi M, Hoshino K, Kaisho T, Takemoto K, Suzuki T, Kuranaga E, Ohara O, Miura M.
Single-cell imaging of caspase-1 dynamics reveals an all-or-none inflammasome signaling response.
Cell Rep 8(4) 974-82(2014) 25127135

Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, Abe K.
Molecular Identification of t(w5): Vps52 Promotes Pluripotential Cell Differentiation through Cell-Cell Interactions.
Cell Rep (2012) 23142660

Oba D, Inoue SI, Miyagawa-Tomita S, Nakashima Y, Niihori T, Yamaguchi S, Matsubara Y, Aoki Y.
Mice with an Oncogenic HRAS Mutation are Resistant to High-Fat Diet-Induced Obesity and Exhibit Impaired Hepatic Energy Homeostasis.
EBioMedicine (2017) 29254681

Nishibe R, Watanabe W, Ueda T, Yamasaki N, Koller R, Wolff L, Honda ZI, Ohtsubo M, Honda H.
CIZ1, a p21<sup>Cip1/Waf1</sup>-interacting protein, functions as a tumor suppressor in vivo.
FEBS Lett. (2013) 23583447

Inoue SI, Moriya M, Watanabe Y, Miyagawa-Tomita S, Niihori T, Oba D, Ono M, Kure S, Ogura T, Matsubara Y, Aoki Y.
New BRAF knock-in mice provide a pathogenetic mechanism of developmental defects and a therapeutic approach in cardio-facio-cutaneous syndrome.
Hum. Mol. Genet. (2014) 25035421

Naganuma T, Takagi S, Kanetake T, Kitamura T, Hattori S, Miyakawa T, Sassa T, Kihara A.
Disruption of the Sjögren-Larsson Syndrome Gene Aldh3a2 in Mice Increases Keratinocyte Growth and Retards Skin Barrier Recovery.
J. Biol. Chem. 291(22) 11676-88. (2016) 27053112

Kiyozumi D, Takeichi M, Nakano I, Sato Y, Fukuda T, Sekiguchi K.
Basement membrane assembly of the integrin alpha 8 beta1 ligand nephronectin requires Fraser syndrome-associated proteins.
J. Cell Biol. 197(5) 677-89(2012) 22613833

Kiyozumi D, Taniguchi Y, Nakano I, Toga J, Yagi E, Hasuwa H, Ikawa M, Sekiguchi K.
Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality.
Life Sci Alliance (2018) 30456378

Ogoh H, Tanuma N, Matsui Y, Hayakawa N, Inagaki A, Sumiyoshi M, Momoi Y, Kishimoto A, Suzuki M, Sasaki N, Ohuchi T, Nomura M, Teruya Y, Yasuda K, Watanabe T, Shima H.
The protein phosphatase 6 catalytic subunit (Ppp6c) is indispensable for proper post-implantation embryogenesis.
Mech. Dev. (2016) 26868000

Sassa T, Ohno Y, Suzuki S, Nomura T, Nishioka C, Kashiwagi T, Hirayama T, Akiyama M, Taguchi R, Shimizu H, Itohara S, Kihara A.
Impaired epidermal permeability barrier in mice lacking the Elovl1 gene responsible for very long-chain fatty acid production.
Mol. Cell. Biol. (2013) 23689133

Ueda T, Nakata Y, Yamasaki N, Oda H, Sentani K, Kanai A, Onishi N, Ikeda K, Sera Y, Honda ZI, Tanaka K, Sata M, Ogawa S, Yasui W, Saya H, Takita J, Honda H.
ALKR1275Q perturbs extracellular matrix, enhances cell invasion and leads to the development of neuroblastoma in cooperation with MYCN.
Oncogene Feb 1. doi: 10.1038/onc.2015.519. (2016) 26829053

Ueda T, Nakata Y, Nagamachi A, Yamasaki N, Kanai A, Sera Y, Sasaki M, Matsui H, Honda ZI, Oda H, Wolff L, Inaba T, Honda H.
Propagation of trimethylated H3K27 regulated by polycomb protein EED is required for embryogenesis, hematopoietic maintenance, and tumor suppression
Proc. Natl. Acad. Sci. U.S.A. (2016) 27578866

Kobayashi M, Sabouri Z, Sabouri S, Kitawaki Y, Pommier Y, Abe T, Kiyonari H, Honjo T.
Decrease in topoisomerase I is responsible for activation-induced cytidine deaminase (AID)-dependent somatic hypermutation.
Proc. Natl. Acad. Sci. U.S.A. 108(48) 19305-10(2011) 22080610

Tamaki T, Uchiyama Y, Okada Y, Tono K, Masuda M, Nitta M, Hoshi A, Akatsuka A.
Clonal differentiation of skeletal muscle-derived CD34(-)/45(-) stem cells into cardiomyocytes in vivo.
Stem Cells Dev. 19(4) 503-12(2010) 19634996