RBRC01817

Information1

Image
BRC No.RBRC01817
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Drd4<tm1(IL2RA)Tao>/KobaRbrc
Former Common nameD4R-IL-2R
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998.
Strain descriptionB6.129P2-Drd4<tm1(IL2RA)Tao. The human interleukin-2 receptor alpha-subunit gene and neo cassettes were introduced into exon 2 of mouse D4R gene. Specific neurons expressing D4R gene of this mice are disrupted by a recombinant immunotoxin.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesJ. Neurosci. 23, 11662-11672 (2003). 14684868

Health Report

Examination Date / Room / Rack2007/07/17Room:4-ARack:D
2007/05/22Room:4-ARack:D
2007/05/21Room:4-ARack:D
2007/03/19Room:4-ARack:D
2007/01/15Room:4-ARack:D
2006/11/13Room:4-ARack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Drd4dopamine receptor 47Drd4<tm1(IL2RA)Tao>targeted mutation 1, Toshihiko Aosaki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyA7HBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IL2RAInterleukin-2 receptor α-subunit (human)7IL2RA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA7

Information2

Donor DNAmouse D4R genomic DNA, human interleukin-2 receptor α subunit, rabbit β globin poly A signal, mouse phosphoglycerate kinase-1 promoter, E. coli neo, SV40 poly A signal
Research applicationNeurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dopamine D4 receptor-induced postsynaptic inhibition of GABAergic currents in mouse globus pallidus neurons. Shin R-M, Masuda M, Miura M, Sano H, Shirasawa T, Song WJ, Kobayashi K, Aosaki T. J. Neurosci. 23 (37) 11662-11672 (2003). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP (Japanese)

BRC mice in Publications

No Data