Strain Data Sheet

RBRC01817

Strain Information

Image
BRC No.RBRC01817
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Drd4<tm1(IL2RA)Tao>/KobaRbrc
Former Common nameD4R-IL-2R
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998.
Strain descriptionB6.129P2-Drd4<tm1(IL2RA)Tao. The human interleukin-2 receptor alpha-subunit gene and neo cassettes were introduced into exon 2 of mouse D4R gene. Specific neurons expressing D4R gene of this mice are disrupted by a recombinant immunotoxin.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References
Dopamine D4 receptor-induced postsynaptic inhibition of GABAergic currents in mouse globus pallidus neurons.
Shin R M, Masuda M, Miura M, Sano H, Shirasawa T, Song W J, Kobayashi K, Aosaki T
J. Neurosci. 23, 11662-11672 (2003). 14684868

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA7
  • attention deficit-hyperactivity disorder(MedGEN)
    • Drd4dopamine receptor D47Drd4targeted mutation 1, Toshihiko Aosaki
  • attention deficit-hyperactivity disorder(MedGEN)
    • HBB2rabbit beta-globin polyA7HBB2
  • attention deficit-hyperactivity disorder(MedGEN)
    • IL2RAInterleukin-2 receptor α-subunit (human)7IL2RA
  • attention deficit-hyperactivity disorder(MedGEN)
    • neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)
  • attention deficit-hyperactivity disorder(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal single cell response(MP:0003463)
    • Detailed phenotype dataPhenopub Link

    Ordering Information

    Donor DNAmouse D4R genomic DNA, human interleukin-2 receptor α subunit, rabbit β globin poly A signal, mouse phosphoglycerate kinase-1 promoter, E. coli neo, SV40 poly A signal
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci. 23 (37) 11662-11672 (2003). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorKazuto Kobayashi (Fukushima Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data