Strain Data Sheet

RBRC01814

Strain Information

Image
BRC No.RBRC01814
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Grin2b<tm1Mim>/MimRbrc
Former Common nameGluRε2 KO mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Masayoshi Mishina at Tokyo University in 1995. A pgk-neo was transfered into TT2 ES cells to replace the exon encoding the transiation initiation site of Grin2b gene. The Mint deficient mice were backcrossed to C57BL/6N for 14 times.
Strain descriptionB6.Cg-Grin2b<tm1Mim>. The Grin2b gene is one of the glutamate recepoter channel subunit families forming NMDA receptor channel, which is important for synaptic plasticity. Homozygous mutant mice show impairments in suckling and die shortly after birth.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NJcl)
References
Roles of the glutamate receptor epsilon2 and delta2 subunits in the potentiation and prepulse inhibition of the acoustic startle reflex.
Takeuchi T, Kiyama Y, Nakamura K, Tsujita M, Matsuda I, Mori H, Munemoto Y, Kuriyama H, Natsume R, Sakimura K, Mishina M
Eur. J. Neurosci., 14, 153-160 (2001). 11488959

Impairment of suckling response, trigeminal neuronal pattern formation, and hippocampal LTD in NMDA receptor epsilon 2 subunit mutant mice.
Kutsuwada T, Sakimura K, Manabe T, Takayama C, Katakura N, Kushiya E, Natsume R, Watanabe M, Inoue Y, Yagi T, Aizawa S, Arakawa M, Takahashi T, Nakamura Y, Mori H, Mishina M
Neuron, 16, 333-344 (1996). 8789948

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grin2bglutamate receptor, ionotropic, NMDA2B (epsilon 2)6Grin2btargeted mutation 1, Masayoshi Mishina
  • developmental and epileptic encephalopathy, 27(MedGEN)

  • intellectual disability, autosomal dominant 6(MedGEN)
    • neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)
  • developmental and epileptic encephalopathy, 27(MedGEN)

  • intellectual disability, autosomal dominant 6(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal AMPA-mediated synaptic currents(MP:0002885)

  • abnormal hippocampus neuron morphology(MP:0009939)

  • absence of NMDA-mediated synaptic currents(MP:0001901)

  • absent gastric milk in neonates(MP:0009546)

  • absent long-term depression(MP:0001899)
    • more 6 phenotypes
  • absent mesencephalic trigeminal nucleus(MP:0000908)

  • absent suckling reflex(MP:0001435)

  • disorganized barrel cortex(MP:0000861)

  • increased prepulse inhibition(MP:0009141)

  • increased startle reflex(MP:0001488)

  • neonatal lethality, complete penetrance(MP:0011087)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse PGK promoter, E. coli neo, mouse GluRε2 subunit genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 16 ,333-344 (1996).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.
    DepositorMasayoshi Mishina (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Tomiyama K, Kato R, Hara Y, Kobayashi M, Mishina M, Yanagawa Y, Kinsella A, Koshikawa N, Waddington JL.
    Phenotypic characterization of orofacial movement topography in mutants with disruption of amino acid mechanisms: glutamate N2A/B/D [GluRε1/2/4] subtypes and the GABA synthesizing enzyme GAD65.
    Neuroscience 250 743-54(2013) 23892010