RBRC01814

Information1

Image
BRC No.RBRC01814
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Grin2b<tm1Mim>/MimRbrc
Former Common nameGluRε2 KO mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Masayoshi Mishina at Tokyo University in 1995. A pgk-neo was transfered into TT2 ES cells to replace the exon encoding the transiation initiation site of Grin2b gene. The Mint deficient mice were backcrossed to C57BL/6N for 14 times.
Strain descriptionB6.Cg-Grin2b<tm1Mim>. The Grin2b gene is one of the glutamate recepoter channel subunit families forming NMDA receptor channel, which is important for synaptic plasticity. Homozygous mutant mice show impairments in suckling and die shortly after birth.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NJcl)
ReferencesNeuron, 16, 333-344 (1996). 8789948
Eur. J. Neurosci., 14, 153-160 (2001). 11488959

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Grin2bglutamate receptor, ionotropic, NMDA2B (epsilon 2)6Grin2b<tm1Mim>targeted mutation 1, Masayoshi Mishina

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse PGK promoter, E. coli neo, mouse GluRε2 subunit genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 16 ,333-344 (1996).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.
DepositorMasayoshi Mishina (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Tomiyama K, Kato R, Hara Y, Kobayashi M, Mishina M, Yanagawa Y, Kinsella A, Koshikawa N, Waddington JL.
Phenotypic characterization of orofacial movement topography in mutants with disruption of amino acid mechanisms: Glutamate N2A/B/D [GluR 1/2/4] subtypes and the GABA synthesizing enzyme GAD65.
Neuroscience 250 743-54(2013) 23892010