Strain Data Sheet

RBRC01813

Strain Information

Image
BRC No.RBRC01813
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Grin2a<tm1Mim>/MimRbrc
Former Common nameGluRε1 KO mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Masayoshi Mishina at Tokyo University in 1995. The targeting vector containing pgk-neo cassette was transferred into TT2 ES cells to replace the exon encoding M2 and M3 transmembrane regions of Grin2a gene. The Grin2a deficient mice were backcrossed to C57BL/6N for 14 times.
Strain descriptionB6.Cg-Grin2a<tm1Mim>. The Grin2a gene is one of the glutamate receptor channel subunit families forming NMDA receptor channel, which is important for synaptic plasticity. The Grin2a homozygous mutant mice show a moderate deficiency in spatial learning. Reproductive performance and growth of them is normal.
Colony maintenancebackcross to C57BL/6 (Heterozygote x C57BL/6NJcl)
References
Increased thresholds for long-term potentiation and contextual learning in mice lacking the NMDA-type glutamate receptor epsilon1 subunit.
Kiyama Y, Manabe T, Sakimura K, Kawakami F, Mori H, Mishina M
J. Neurosci., 18, 6704-6712 (1998). 9712642

Reduced hippocampal LTP and spatial learning in mice lacking NMDA receptor epsilon 1 subunit.
Sakimura K, Kutsuwada T, Ito I, Manabe T, Takayama C, Kushiya E, Yagi T, Aizawa S, Inoue Y, Sugiyama H, et a l
Nature, 373, 151-155 (1995). 7816096

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grin2aglutamate receptor, ionotropic, NMDA2A (epsilon 1)16Grin2atargeted mutation 1, Masayoshi Mishima
  • Landau-Kleffner syndrome(MedGEN)
    • neoneomycin resistance gene (E. coli)16mouse phosphoglycerate kinase promoter (PGK promoter)
  • Landau-Kleffner syndrome(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal spatial learning(MP:0001463)

  • increased startle reflex(MP:0001488)

  • jumpy(MP:0001401)

  • reduced NMDA-mediated synaptic currents(MP:0001902)

  • reduced long-term potentiation(MP:0001473)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse PGK promoter, E. coli neo, mouse GluRε1 subunit genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 373, 151-155 (1995).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.
    DepositorMasayoshi Mishina (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Tomiyama K, Kato R, Hara Y, Kobayashi M, Mishina M, Yanagawa Y, Kinsella A, Koshikawa N, Waddington JL.
    Phenotypic characterization of orofacial movement topography in mutants with disruption of amino acid mechanisms: glutamate N2A/B/D [GluRε1/2/4] subtypes and the GABA synthesizing enzyme GAD65.
    Neuroscience 250 743-54(2013) 23892010