RBRC01774

Information1

Image
BRC No.RBRC01774
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Ep400<tm1Fuku>/FukuRbrc
Former Common namemDom-KO, mDomino knockout mouse
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Rikiro Fukunaga at Osaka University in 2003. Part of exon 2 and inron 2 was replaced with a neomycin selection cassette. The mutant mice were backcrossed to C57BL/6.
Strain descriptionB6.129-Ep400<tm1Fuku>. p400/mDomino (mammalian Domino) gene is an Swr1-class DNA-dependent ATPase that is a core subunit of a large chromatin-remodeling complex, and is currently suggested to play a unique function in histone variant exchange. The mDomino deficient mice are embryonic lethal and show anemic in appearance and slight deformity of the neural tubes.
Colony maintenanceBackcross to C57BL/6 Backcross to C57BL/6 (Heterozygote x C57BL/6N)
ReferencesGenes Cells, 12, 581-592 (2007). 17535249

Health Report

Examination Date / Room / Rack2008/05/20Room:4-ARack:H
2008/05/19Room:4-ARack:H
2008/03/17Room:4-ARack:H
2008/01/14Room:4-ARack:H
2007/11/12Room:4-ARack:H
2007/09/18Room:4-ARack:H
2007/07/17Room:4-ARack:H
2007/05/22Room:4-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ep400E1A binding protein p4005Ep400<tm1Fuku>targeted mutation 1, Rikiro Fukunaga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase-1 promoter, E. coli neo, mouse mDomino genomic DNA
Research applicationDevelopmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes to Cells, 12, 581-592 (2007).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author.
DepositorRikiro Fukunaga (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Contact Information (Japanese)
Genotyping protocol -PCR-
Mouse of the Month Jan 2008

BRC mice in Publications

Chevillard-Briet M, Quaranta M, Gr zy A, Mattera L, Courilleau C, Philippe M, Mercier P, Corpet D, Lough J, Ueda T, Fukunaga R, Trouche D, Escaffit F.
Interplay between chromatin modifying enzymes controls colon cancer progression through Wnt signaling.
Hum. Mol. Genet. (2013) 24287617