Strain Data Sheet

RBRC01772

Strain Information

Image
BRC No.RBRC01772
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Tmbim1<tm1Noj>/41
Former Common nameRECS1 KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Hanjun Zhao, Norikazu Yabuta and Hiroshi Nojima, Research Institute for Microbial Diseases, Osaka University in 2001. D3 ES cells were used to generate the knockout mice. The mutant mice were backcrossed to C57BL/6 over 8 times.
Strain descriptionRECS1 (Tmbim1) gene knockout mice. The 5’-untranslated sequence (-3.8 kb) and exon 1-6 region of RECS1 was replaced by PGK-neo cassette. Homozygous mutant mice are prone to aortic dilation and cystic medial degeneration (CMD) when the mice ages (older than 14 months). Aortic dilation and CMD are frequently encountered. Viable and fertile.
Colony maintenanceHeterozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
References
RECS1 deficiency in mice induces susceptibility to cystic medial degeneration.
Zhao H, Ito A, Kimura S H, Yabuta N, Sakai N, Ikawa M, Okabe M, Matsuzawa Y, Yamashita S, Nojima H
Genes Genet Syst., 81, 41-50 (2006). 16607040

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tmbim1transmembrane BAX inhibitor motif containing 11Tmbim1targeted mutation 1, Hiroshi Nojima
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal aorta tunica media morphology(MP:0009873)

  • abnormal circulating enzyme level(MP:0001570)

  • abnormal enzyme/coenzyme activity(MP:0005584)

  • cardiovascular system phenotype(MP:0005385)

  • dilated aorta(MP:0010574)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Tmbim1 genomic DNA
    Research applicationCardiovascular Research
    Mouse Models for Human Disease
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECS1 deficiency in mice induces susceptibility to cystic medical degeneration. Hanjun Zhao et al. (2006) Genes Genet. Syst. 81, p41-50The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorHiroshi Nojima (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jan 2007

    BRC mice in Publications

    No Data