BRC No.RBRC01769
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus domesticus
Strain name129(B6)-Zfp318<tm1>
Former Common nameTZF KO (129), 129-TZF KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain129/SvJ
Strain developmentDeveloped by Hiromi Hagiwara, Faculty of Biomedical Engineering, Toin University of Yokohama in 2005.
Strain descriptionTZF (Zfp318) gene knockout mice. Homozygous males of C57BL/6 background (RBRC01768) have a tendency to exhibit infertile. Homozygous males of 129 background (RBRC01769) are infertile.
Colony maintenanceHeterozygote x Wild-type [or Crossing to 129X1/SvJJmsSlc]
ReferencesBiochem. Biophys. Res. Commun., 273, 398-403 (2000). 10873617
Proc. Natl. Acad. Sci. USA, 111, 4513-4518 (2014). 24616512
Biochem. Biophys. Res. Commun., 341, 515-521 (2006). 16446156
Biochem. Biophys. Res. Commun., 331, 1025-1031 (2005). 15882980
Mol. Cell. Endocrinology, 247, 150-165 (2006). 16469430
Biochem. Biophys. Res. Commun., 301, 1079-1085 (2003). 12589823

Health Report

Examination Date / Room / Rack2019/10/16Room:3-3Rack:A


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Zfp318zinc finger protein 31817Zfp318

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)17

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)17herpes simplex virus thymidine kinase promoter (HSV tk promoter)


Donor DNAmouse Zfp318, E. coli neomycin resistance gene, Jellyfish GFP cDNA, HSV tk promoter
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorHiromi Hagiwara (Toin University of Yokohama)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)

BRC mice in Publications

No Data