Strain Data Sheet

RBRC01768

Strain Information

Image
BRC No.RBRC01768
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;129-Zfp318<tm1Hhag>
Former Common nameTZF KO (B6), B6-TZK KO, B6;129-Zfp318<tm1>/Rbrc
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Hiromi Hagiwara, Faculty of Biomedical Engineering, Toin University of Yokohama in 2005.
Strain descriptionTZF (Zfp318) gene knockout mice. Homozygous males of C57BL/6 background (RBRC01768) have a tendency to exhibit infertile. Homozygous males of 129 background (RBRC01769) are infertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
References
The transcript for a novel protein with a zinc finger motif is expressed at specific stages of mouse spermatogenesis.
Inoue A, Ishiji A, Kasagi S, Ishizuka M, Hirose S, Baba T, Hagiwara H
Biochem. Biophys. Res. Commun., 273, 398-403 (2000). 10873617

Molecular cloning and characteristics of a novel zinc finger protein and its splice variant whose transcripts are expressed during spermatogenesis.
Ishizuka M, Ohshima H, Tamura N, Nakada T, Inoue A, Hirose S, Hagiwara H
Biochem. Biophys. Res. Commun., 301, 1079-1085 (2003). 12589823

A zinc finger protein TZF is a novel corepressor of androgen receptor.
Ishizuka M, Kawate H, Takayanagi R, Ohshima H, Tao R H, Hagiwara H
Biochem. Biophys. Res. Commun., 331, 1025-1031 (2005). 15882980

Testicular zinc finger protein recruits histone deacetylase 2 and suppresses the transactivation function and intranuclear foci formation of agonist-bound androgen receptor competitively with TIF2.
Tao R H, Kawate H, Wu Y, Ohnaka K, Ishizuka M, Inoue A, Hagiwara H, Takayanagi R
Mol. Cell. Endocrinology, 247, 150-165 (2006). 16469430

Zinc-finger protein ZFP318 is essential for expression of IgD, the alternatively spliced Igh product made by mature B lymphocytes.
Enders A, Short A, Miosge L A, Bergmann H, Sontani Y, Bertram E M, Whittle B, Balakishnan B, Yoshida K, Sjollema G, Field M A, Andrews T D, Hagiwara H, Goodnow C C
Proc. Natl. Acad. Sci. USA, 111, 4513-4518 (2014). 24616512

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)17
Zfp318zinc finger protein 31817Zfp318targeted mutation 1, Hiromi Hagiwara
neoneomycin resistance gene (E. coli)17herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased IgD level(MP:0002491)

  • hematopoietic system phenotype(MP:0005397)

  • increased IgM level(MP:0002494)

  • male infertility(MP:0001925)
    • Detailed phenotype data

    Ordering Information

    Donor DNAJellyfish GFP cDNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk poly A, mouse TZF genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorHiromi Hagiwara (Toin University of Yokohama)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data