Strain Data Sheet

RBRC01767

Strain Information

Image
BRC No.RBRC01767
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S4-Tob1<tm1Tya> Tob2<tm1Tya>/TyaRbrc
Former Common nametob and tob2 KO (B6)
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Dr. Tetsuo Noda, Japanese Foundation for Cancer Research Cancer Institute at and Dr. Tadashi Yamamoto at the University of Tokyo. This strain was produced by crossing Tob1 KO mice and Tob2 KO mice.
Strain descriptionTob is a member of antiproliferative protein family which is a negative regulator BMP/Smad signaling in osteoblasts. Tob1 null mice are viable and exhibit bone mass due to increased numbers of osteoblasts. While, Tob2 null mice appear normal. This strain is useful for elucidating of Tob gene function.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tob1transducer of ErbB-2.111Tob1targeted mutation 1, Tadashi Yamamoto
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)
Tob2transducer of ERBB2, 215Tob2targeted mutation 1, Tadashi Yamamoto
lacZbeta-galactosidase (E. coli)15

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal bone ossification(MP:0008271)

  • abnormal osteoblast differentiation(MP:0008395)

  • abnormal osteoclast differentiation(MP:0008396)

  • abnormal osteoclast physiology(MP:0001541)

  • abnormal trabecular bone morphology(MP:0000130)
    • more 8 phenotypes
  • decreased bone mass(MP:0004016)

  • increased bone mass(MP:0005605)

  • increased bone resorption(MP:0004992)

  • increased cell proliferation(MP:0000351)

  • increased osteoblast cell number(MP:0004988)

  • increased osteoclast cell number(MP:0004984)

  • increased trabecular bone mass(MP:0010872)

  • osteoporosis(MP:0000066)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli neo, mouse PGK promoter, mouse tob genomic DNA, E. coli LacZ, mouse tob2 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorTadashi Yamamoto (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data