Strain Data Sheet

RBRC01766

Strain Information

Image
BRC No.RBRC01766
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S4-Tob2<tm1Tya>/TyaRbrc
Former Common nametob2-gene KO (B6)
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Tetsuo Noda, Japanese Foundation for Cancer Research Cancer Institute at and Dr. Tadashi Yamamoto at the University of Tokyo in 1999. LacZ was inserted into Tob2 gene. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionB6.129S4-Tob2<tm1Tya>. Tob gene is a member of antiproliferative protein family which is a negative regulator BMP/Smad signaling in osteoblasts. Tob2 null mice are viable and appear normal. This strain is useful for elucidating of Tob gene function.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References
Cloning and characterization of the mouse tob2 gene.
Ajima R, Ikematsu N, Ohsugi M, Yoshida Y, Yamamoto T
Gene, 253, 215-220 (2000). 10940559

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tob2transducer of ERBB2, 215Tob2targeted mutation 1, Tadashi Yamamoto
lacZbeta-galactosidase (E. coli)15
loxPphage P1 loxP15loxP
nlsSV40 large T antigen nuclear localization signal (NLS)15nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal osteoclast differentiation(MP:0008396)

  • abnormal osteoclast physiology(MP:0001541)

  • abnormal trabecular bone morphology(MP:0000130)

  • decreased bone mass(MP:0004016)

  • increased bone resorption(MP:0004992)
    • more 2 phenotypes
  • increased osteoclast cell number(MP:0004984)

  • osteoporosis(MP:0000066)
    • Detailed phenotype data

    Ordering Information

    Donor DNASV40 large T antigen nuclear localization signal (NLS), E. coli LacZ, pA, phage P1 loxP site, mouse Tob2 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorTadashi Yamamoto (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data