RBRC01765

Information1

Image
BRC No.RBRC01765
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S4-Tob1<tm1Tya>/TyaRbrc
Former Common nametob-gene KO (B6)
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Tetsuo Noda at Japanese Foundation for Cancer Research Cancer Institute and Dr. Tadashi Yamamoto at the University of Tokyo in 1997. A PGK-neo cassette was inserted into the cording region of Tob1 gene. C57BL/6J background.
Strain descriptionTob is a member of antiproliferative protein family which is a negative regulator BMP/Smad signaling in osteoblasts. Tob1 null mice are viable and exhibit bone mass due to increased numbers of osteoblasts. This strain is useful for elucidating of Tob gene function.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesCell, 103, 1085-1097 (2000). 11163184
Genes Dev., 17, 1201-1206 (2003). 12756225

Health Report

Examination Date / Room / Rack2008/03/17Room:4-ARack:F
2008/01/14Room:4-ARack:F
2007/11/12Room:4-ARack:F
2007/09/18Room:4-ARack:F
2007/07/17Room:4-ARack:F
2007/05/22Room:4-ARack:F
2007/05/21Room:4-ARack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tob1transducer of ErbB-2.111Tob1<tm1Tya>targeted mutation 1, Tadashi Yamamoto

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), mouse tob genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorTadashi Yamamoto (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Schulze-Topphoff U, Casazza S, Varrin-Doyer M, Pekarek K, Sobel RA, Hauser SL, Oksenberg JR, Zamvil SS, Baranzini SE.
Tob1 plays a critical role in the activation of encephalitogenic T cells in CNS autoimmunity.
J. Exp. Med. (2013) 23797093

Didonna A, Cekanaviciute E, Oksenberg JR, Baranzini SE.
Immune cell-specific transcriptional profiling highlights distinct molecular pathways controlled by Tob1 upon experimental autoimmune encephalomyelitis.
Sci Rep (2016) 27546286